Gupta R N, Stefanec M, Eng F
Clin Biochem. 1983 Apr;16(2):94-7. doi: 10.1016/s0009-9120(83)90614-8.
A universal gas chromatographic procedure for the determination of the commonly prescribed tricyclic antidepressant drugs in 1 mL of plasma is described. After the addition of internal standards the samples are washed with pentane at acidic pH and extracted with pentane at alkaline pH. The pentane extract is evaporated after the addition of acetic anhydride. Aliquots of a methanolic solution of the residue of evaporated extract are analyzed isothermally on a Durabond 30 m fused silica column. This column is well suited for use with a nitrogen selective detector. The liquid phase is not extracted by repeated injections of methanolic solutions of plasma extracts. When compared to a packed column, this column allows shorter analysis time.
本文描述了一种通用的气相色谱法，用于测定1 mL血浆中常用的三环类抗抑郁药物。加入内标后，样品在酸性pH下用戊烷洗涤，然后在碱性pH下用戊烷萃取。加入乙酸酐后，将戊烷萃取液蒸发。将蒸发萃取物残渣的甲醇溶液等分试样在一根30 m的Durabond熔融石英柱上进行等温分析。该柱非常适合与氮选择性检测器联用。通过重复进样血浆萃取物的甲醇溶液，不会萃取液相。与填充柱相比，该柱可缩短分析时间。
