Maruyama I, Yamamoto T, Ochi J, Nakai Y, Yamada S
Eur J Pharmacol. 1983 Jun 17;90(4):325-31. doi: 10.1016/0014-2999(83)90553-8.
The effect of dopamine on ciliary movement and the existence of dopamine-containing cells in the ciliated epithelium of frog palatine mucosa were investigated pharmacologically and by means of electron and fluorescence microscopy, respectively. Ciliary movement was suppressed markedly when the perfusion medium contained 10(-6) M dopamine, thereby indicating that dopamine has a suppressive effect on ciliary movement. Electron microscopy revealed at least two types of granule-containing (GC) cells, type 1 cells containing small spherical granular vesicles (about 100-150 nm in diameter) and type 2 cells containing elongated dense granules (about 150 X 250 nm in diameter). One hour after systemic administration of dihydroxyphenylalanine-[3H(G)] [( 3H]DOPA), a large number of silver grains appeared only in type 2 GC cells, thus indicating that type 2 cells are catecholamine-storing. Blue-green fluorescent cells were detected in the ciliated epithelium. On microspectrofluorometry, catecholamine fluorescence in the cells showed a main excitation and emission maxima at 415 and 480 nm, respectively. When the fluorescent cells were exposed to HCl vapor for several s, the excitation peak shifted to 380 nm and this peak was unchanged after treatment for an additional 5 min. It may be considered that these fluorescent cells contain dopamine and correspond to type 2 GC cells. A possible functional relationship between regulation of ciliary movement and dopamine-containing cells was also discussed.
分别采用药理学方法以及电子显微镜和荧光显微镜技术，研究了多巴胺对青蛙腭黏膜纤毛运动的影响以及纤毛上皮中含多巴胺细胞的存在情况。当灌注液中含有10⁻⁶M多巴胺时，纤毛运动明显受到抑制，这表明多巴胺对纤毛运动具有抑制作用。电子显微镜显示至少有两种含颗粒（GC）细胞，1型细胞含有小的球形颗粒囊泡（直径约100 - 150nm），2型细胞含有细长的致密颗粒（直径约150×250nm）。全身给予二羟基苯丙氨酸-[³H(G)]（[³H]多巴）1小时后，大量银颗粒仅出现在2型GC细胞中，这表明2型细胞储存儿茶酚胺。在纤毛上皮中检测到蓝绿色荧光细胞。在显微分光荧光测定中，细胞中的儿茶酚胺荧光分别在415和480nm处显示出主要的激发和发射最大值。当荧光细胞暴露于HCl蒸汽中数秒时，激发峰移至380nm，再处理5分钟后该峰不变。可以认为这些荧光细胞含有多巴胺，并且对应于2型GC细胞。还讨论了纤毛运动调节与含多巴胺细胞之间可能的功能关系。
