Maruyama I
Eur J Pharmacol. 1984 Jan 27;97(3-4):239-45. doi: 10.1016/0014-2999(84)90455-2.
The effects of noradrenaline on ciliary movement of frog palatine mucosa were investigated using the particle transport method. Prostaglandins and cyclic AMP concentrations in the mucosa were determined by radioimmunoassay. Concentrations of noradrenaline below 10(-7) M suppressed ciliary movement by about 40% as compared to the control level. The changeover from inhibition to acceleration of ciliary movement occurred at 10(-7) M noradrenaline. Ciliary movement was markedly accelerated by concentrations of noradrenaline over 2 X 10(-6) M. This acceleration was eliminated in the presence of inhibitors of prostaglandin biosynthesis such as indomethacin (14 microM) and aspirin (25 microM). The acceleration was not eliminated by an inhibitor of thromboxane A2 biosynthesis (OKY-1555). On the other hand, E type prostaglandin release was increased about 8 fold of control by treatment with 5 X 10(-5) M noradrenaline, while F type prostaglandin release was not affected by the treatment with 5 X 10(-5) M noradrenaline. Ciliary movement was markedly accelerated when prostaglandin E1 was administered. The concentration of cyclic AMP was increased about 2.1 fold by treatment with 5 X 10(-5) M noradrenaline. The possible mechanism of the effects of noradrenaline on ciliary movement is discussed in the light of these results.
采用颗粒转运法研究了去甲肾上腺素对蛙腭黏膜纤毛运动的影响。通过放射免疫分析法测定黏膜中前列腺素和环磷酸腺苷的浓度。与对照水平相比，浓度低于10(-7) M的去甲肾上腺素可使纤毛运动抑制约40%。在10(-7) M去甲肾上腺素时，纤毛运动从抑制转变为加速。浓度超过2×10(-6) M的去甲肾上腺素可使纤毛运动明显加速。在存在前列腺素生物合成抑制剂如吲哚美辛（14 microM）和阿司匹林（25 microM）的情况下，这种加速作用消失。血栓素A2生物合成抑制剂（OKY-1555）并未消除这种加速作用。另一方面，用5×10(-5) M去甲肾上腺素处理后，E型前列腺素的释放量增加至对照水平的约8倍，而F型前列腺素的释放不受5×10(-5) M去甲肾上腺素处理的影响。给予前列腺素E1时，纤毛运动明显加速。用5×10(-5) M去甲肾上腺素处理后，环磷酸腺苷的浓度增加约2.1倍。根据这些结果讨论了去甲肾上腺素对纤毛运动影响的可能机制。
