Aivasashvilli V A, Beabealashvilli R S
FEBS Lett. 1983 Aug 22;160(1-2):124-8. doi: 10.1016/0014-5793(83)80950-8.
We have developed a method to localize specific sites where RNA elongation is arrested due to DNA-bound ligands. The method was used to determine apparent binding sites for actinomycin D. We have found 14 strong RNA hindrance sites along nucleotide sequence of T7 and D111 T7 DNA of 380 nucleotides full length under low actinomycin D concentration conditions. Nucleotide sequence of all the sites is described by general formula XGCY where X not equal to G and Y not equal to C.
我们开发了一种方法来定位由于与DNA结合的配体而导致RNA延伸停滞的特定位点。该方法用于确定放线菌素D的表观结合位点。在低浓度放线菌素D条件下，我们在全长380个核苷酸的T7和D111 T7 DNA的核苷酸序列中发现了14个强烈的RNA阻碍位点。所有位点的核苷酸序列由通式XGCY描述，其中X不等于G且Y不等于C。
