嗜热脂肪芽孢杆菌起始因子IF3的一级结构。
The primary structure of initiation factor IF3 from Bacillus stearothermophilus.
作者信息
Kimura M, Ernst H, Appelt K
出版信息
FEBS Lett. 1983 Aug 22;160(1-2):78-81. doi: 10.1016/0014-5793(83)80940-5.
The complete amino acid sequence of the initiation factor IF3 from Bacillus stearothermophilus has been elucidated. This was achieved by splitting the protein with trypsin, Staphylococcus protease or cyanogen bromide. The amino acid sequence was determined by manual Edman degradation, using the DABITC/PITC double-coupling method. The IF3 molecule contains 171 amino acids and has an Mr of 19 677. The sequence was compared to the homologous molecule from Escherichia coli; about 50% of the amino acid residues were found to be identical.
嗜热脂肪芽孢杆菌起始因子IF3的完整氨基酸序列已被阐明。这是通过用胰蛋白酶、葡萄球菌蛋白酶或溴化氰裂解该蛋白质来实现的。氨基酸序列采用DABITC/PITC双偶联法通过手动埃德曼降解法测定。IF3分子含有171个氨基酸，Mr为19677。将该序列与大肠杆菌的同源分子进行比较；发现约50%的氨基酸残基是相同的。
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