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嗜热脂肪芽孢杆菌中包含infC、rpmI和rplT的一个基因簇的克隆与特性分析

Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT.

作者信息

Pon C L, Brombach M, Thamm S, Gualerzi C O

机构信息

Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin.

出版信息

Mol Gen Genet. 1989 Aug;218(2):355-7. doi: 10.1007/BF00331290.

DOI:10.1007/BF00331290
PMID:2779520
Abstract

Using two synthetic deoxyribonucleotide probes encoding segments of the primary structure of initiation factor IF3 from Bacillus stearothermophilus, we identified and cloned a segment of DNA which carries the infC gene. As in Escherichia coli, the infC gene begins with the unusual initiation triplet AUU, and is followed by the structural genes for ribosomal proteins L35 and L20 (rpmI and rplT, respectively).

摘要

我们使用两个合成的脱氧核糖核苷酸探针,它们编码嗜热脂肪芽孢杆菌起始因子IF3一级结构的片段,以此鉴定并克隆了一段携带infC基因的DNA片段。与大肠杆菌一样,infC基因起始于不寻常的起始三联体AUU,随后是核糖体蛋白L35和L20的结构基因(分别为rpmI和rplT)。

相似文献

1
Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT.嗜热脂肪芽孢杆菌中包含infC、rpmI和rplT的一个基因簇的克隆与特性分析
Mol Gen Genet. 1989 Aug;218(2):355-7. doi: 10.1007/BF00331290.
2
Identification of a putative infC-rpmI-rplT operon flanked by long inverted repeats in Mycoplasma fermentans (incognitus strain).在发酵支原体(未确定菌株)中鉴定出一个假定的infC-rpmI-rplT操纵子,其两侧为长反向重复序列。
Gene. 1993 May 15;127(1):79-85. doi: 10.1016/0378-1119(93)90619-e.
3
Messenger RNA secondary structure and translational coupling in the Escherichia coli operon encoding translation initiation factor IF3 and the ribosomal proteins, L35 and L20.在编码翻译起始因子IF3以及核糖体蛋白L35和L20的大肠杆菌操纵子中的信使核糖核酸二级结构与翻译偶联
J Mol Biol. 1992 Nov 20;228(2):366-86. doi: 10.1016/0022-2836(92)90827-7.
4
Translated translational operator in Escherichia coli. Auto-regulation in the infC-rpmI-rplT operon.大肠杆菌中的翻译转译操纵子。infC-rpmI-rplT操纵子中的自我调节。
J Mol Biol. 1990 Jun 5;213(3):465-75. doi: 10.1016/S0022-2836(05)80208-6.
5
In vivo transcriptional pattern in the infC operon of Bacillus stearothermophilus.
Mol Gen Genet. 1991 May;227(1):60-4. doi: 10.1007/BF00260707.
6
Translational regulation of infC operon in Bacillus stearothermophilus.
Biochem Cell Biol. 1995 Nov-Dec;73(11-12):1071-8. doi: 10.1139/o95-115.
7
Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation in Escherichia coli.大肠杆菌中翻译起始因子IF3和核糖体蛋白L20受限的生理效应
Mol Gen Genet. 1996 Apr 10;250(6):705-14. doi: 10.1007/BF02172982.
8
The dsg gene of Myxococcus xanthus encodes a protein similar to translation initiation factor IF3.黄色粘球菌的dsg基因编码一种类似于翻译起始因子IF3的蛋白质。
J Bacteriol. 1994 Mar;176(5):1427-33. doi: 10.1128/jb.176.5.1427-1433.1994.
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Nucleotide sequences of Bacillus stearothermophilus ribosomal protein genes: part of the ribosomal S10 operon.嗜热脂肪芽孢杆菌核糖体蛋白基因的核苷酸序列:核糖体S10操纵子的一部分。
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The nucleotide sequences of Bacillus stearothermophilus ribosomal protein S12 and S7 genes: comparison with the str operon of Escherichia coli.嗜热脂肪芽孢杆菌核糖体蛋白S12和S7基因的核苷酸序列:与大肠杆菌str操纵子的比较。
Agric Biol Chem. 1991 Jan;55(1):207-13. doi: 10.1271/bbb1961.55.207.

引用本文的文献

1
Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation.翻译后修饰的全蛋白质组分析:质谱技术在蛋白质基因组注释中的应用
Genome Res. 2007 Sep;17(9):1362-77. doi: 10.1101/gr.6427907. Epub 2007 Aug 9.
2
Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.枯草芽孢杆菌和大肠杆菌中nusA-infB操纵子的相似组织形式。
J Bacteriol. 1993 May;175(10):2880-7. doi: 10.1128/jb.175.10.2880-2887.1993.
3
Translation of mRNAs with degenerate initiation triplet AUU displays high initiation factor 2 dependence and is subject to initiation factor 3 repression.

本文引用的文献

1
Gene organization around the phenylalanyl-transfer ribonucleic acid synthetase locus in Escherichia coli.大肠杆菌中苯丙氨酰 - 转移核糖核酸合成酶基因座周围的基因组织。
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The primary structure of initiation factor IF3 from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌起始因子IF3的一级结构。
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Escherichia coli translational initiation factor IF3: a unique case of translational regulation.
起始三联体为简并形式AUU的mRNA的翻译表现出对起始因子2的高度依赖性,并受到起始因子3的抑制。
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In vivo transcriptional pattern in the infC operon of Bacillus stearothermophilus.
Mol Gen Genet. 1991 May;227(1):60-4. doi: 10.1007/BF00260707.
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Nucleotide sequence of a region of maize chloroplast DNA containing the 3' end of clpP, exon 1 of rps12 and rpl20 and their cotranscription.玉米叶绿体DNA一个区域的核苷酸序列,该区域包含clpP的3'末端、rps12的外显子1和rpl20以及它们的共转录。
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Molecular cloning and sequence of the Bacillus stearothermophilus translational initiation factor IF2 gene.嗜热脂肪芽孢杆菌翻译起始因子IF2基因的分子克隆与序列分析
Mol Gen Genet. 1986 Oct;205(1):97-102. doi: 10.1007/BF02428037.
6
Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS.大肠杆菌DNA结合蛋白H-NS的结构基因hns的鉴定、克隆、核苷酸序列及染色体图谱定位
Mol Gen Genet. 1988 May;212(2):199-202. doi: 10.1007/BF00334684.
7
Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli.大肠杆菌thrS-infC-rplT操纵子的转录模式。
Gene. 1988 Mar 31;63(2):309-20. doi: 10.1016/0378-1119(88)90534-3.
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The primary structure of protein L20 from the large subunit of the Escherichia coli ribosome.
FEBS Lett. 1979 Jul 1;103(1):61-5. doi: 10.1016/0014-5793(79)81250-8.
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Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.用于大肠杆菌起始因子3基因的特异性转导噬菌体。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3970-4. doi: 10.1073/pnas.74.9.3970.
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A specialized transducing lambda phage carrying the Escherichia coli genes for phenylalanyl-tRNA synthetase.一种携带大肠杆菌苯丙氨酰 - tRNA合成酶基因的特异性转导λ噬菌体。
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