Watkins J B, Järvenpää A L, Szczepanik-Van Leeuwen P, Klein P D, Rassin D K, Gaull G, Räihä N C
Gastroenterology. 1983 Oct;85(4):793-800.
This study was conducted to compare the influence of diet on the physiologic changes in bile acid kinetics, intraluminal bile acid concentrations, conjugation patterns, and nutrient lipid absorption, which occur postnatally. Preterm infants, 31-35 wk gestation, were fed one of four diets: (a) human milk pasteurized at 62 degrees C for 30 min, 55% from a pooled source, 35% from the infant's own mother, with the remainder (approximately 10%) being fresh human milk; (b) an adapted infant formula (F1); (c) F1 supplemented with taurine, 30 mumol/dl, (F2); and (d) F1 with both taurine, 30 mumol/dl, and cholesterol, 9.6 mg/dl, to a total of 12.7 mg/dl, the levels found in human milk (F3). In all infants, the bile acid pool size increased nearly twofold between 11 and 35 days, irrespective of dietary regimens. Taurine conjugation of bile acids predominated in all infants at 11 days of age and at 35 days in those infants fed human milk or the taurine-supplemented formulas. In taurine-supplemented formulas, the conjugation pattern did not influence bile acid kinetics. However, the bile acid pool and intraluminal bile acid concentrations were significantly greater in infants fed human milk at all ages, suggesting that human milk feeding, per se, uniquely influences intestinal and possibly hepatic function independent of developmental factors.
本研究旨在比较饮食对出生后胆汁酸动力学、肠腔内胆汁酸浓度、结合模式以及营养脂质吸收等生理变化的影响。选取妊娠31 - 35周的早产儿，喂食四种饮食之一：(a) 62摄氏度巴氏杀菌30分钟的人乳，55%来自混合奶源，35%来自婴儿自己的母亲，其余（约10%）为新鲜人乳；(b) 一种适配的婴儿配方奶粉（F1）；(c) 添加30 μmol/dl牛磺酸的F1（F2）；(d) 添加30 μmol/dl牛磺酸和9.6 mg/dl胆固醇（总量达12.7 mg/dl，即人乳中发现的水平）的F1（F3）。在所有婴儿中，无论饮食方案如何，胆汁酸池大小在11至35天之间增加了近两倍。11日龄时，所有婴儿胆汁酸的牛磺酸结合均占主导，对于喂食人乳或添加牛磺酸配方奶粉的婴儿，35日龄时也是如此。在添加牛磺酸的配方奶粉中，结合模式不影响胆汁酸动力学。然而，各年龄段喂食人乳的婴儿胆汁酸池和肠腔内胆汁酸浓度均显著更高，这表明母乳喂养本身独特地影响肠道乃至可能的肝脏功能，且不受发育因素影响。
