Natsuume-Sakai S, Moriwaki K, Migita S, Sudo K, Suzuki K, Lu D Y, Wang C, Takahashi M
Immunogenetics. 1983;18(2):117-24. doi: 10.1007/BF00368539.
New alleles of murine factor B (Bf) protein were demonstrated. When ethylenediaminetetraacetic acid (EDTA)-plasmas from inbred and wild mice were analyzed by isoelectrofocusing (IEF) and immunofixation, murine Bf proteins were visualized as distinct protein bands in all mice tested. Four variants of murine Bf could be demonstrated in a large number of tested mice: Bf 1 (isoelectrofocusing point (P.I.) range of 5.8-6.1) exemplified by B10 and B10.BR, Bf 2 (P.I. range of 5.8-6.0) exemplified by B10.MOL (OHM), Bf 3 (P.I. range of 5.6-5.9) exemplified by B10.MOL (TEN2) and Mus musculus (Mus m.) subspecies Chc, Bf 4 (P.I. range of 6.0-6.3) exemplified by Mus m. subspecies Shh. The genetic linkage between S locus and Bf locus was studied with two backcross progenies--[B10.BR X (B10.BR X Mus m. subspecies Chc)F1] and [B10.BR X (B10.BR X Mus m. subspecies Shh)F1]. Totally, 256 backcross progenies were typed for Bf type and for Ss type (plasma level of the fourth complement protein regulated by S locus). The results indicated that murine Bf was controlled by a single codominant locus located close to the H-2 complex because no mouse showing recombination between Bf locus and S locus was found.
已证实了小鼠补体因子B（Bf）蛋白的新等位基因。当通过等电聚焦（IEF）和免疫固定分析近交系和野生小鼠的乙二胺四乙酸（EDTA）血浆时，在所有测试的小鼠中，小鼠Bf蛋白都显示为不同的蛋白条带。在大量测试小鼠中可证实有四种小鼠Bf变体：以B10和B10.BR为例的Bf 1（等电聚焦点（P.I.）范围为5.8 - 6.1），以B10.MOL（OHM）为例的Bf 2（P.I.范围为5.8 - 6.0），以B10.MOL（TEN2）和小家鼠（Mus m.）亚种Chc为例的Bf 3（P.I.范围为5.6 - 5.9），以小家鼠亚种Shh为例的Bf 4（P.I.范围为6.0 - 6.3）。利用两个回交后代——[B10.BR×（B10.BR×小家鼠亚种Chc）F1]和[B10.BR×（B10.BR×小家鼠亚种Shh）F1]研究了S位点与Bf位点之间的遗传连锁。总共对256个回交后代进行了Bf类型和Ss类型（由S位点调节的第四种补体蛋白的血浆水平）的分型。结果表明，小鼠Bf由一个位于H - 2复合体附近的共显性单一位点控制，因为未发现Bf位点与S位点之间发生重组的小鼠。
