Gibbons R J, Etherden I
Infect Immun. 1983 Sep;41(3):1190-6. doi: 10.1128/iai.41.3.1190-1196.1983.
Oral bacteria were found to differ in their surface hydrophobicities as determined by their ability to adsorb to hexadecane. Strains of Actinomyces viscosus, A. naeslundii, Streptococcus sanguis, S. mitis, and Bacteroides gingivalis proved highly hydrophobic. Strains of B. intermedius, S. salivarius, S. mutans, and B. melaninogenicus were less hydrophobic, whereas strains of Actinobacillus actinomycetemcomitans were hydrophilic. An overall correlation was noted between the adsorption of bacteria to hexadecane and their numbers which attached to experimental salivary pellicles formed on hydroxyapatite surfaces. This suggests that hydrophobic bonding plays an important role in this process. Pellicles prepared from saliva which had been extracted with chloroform-methanol to remove lipids adsorbed comparable numbers of S. sanguis and A. viscosus and increased numbers of S. mutans. Analyses of adsorption isotherms indicated that pellicles formed from lipid-depleted saliva contained increased numbers of binding sites for the S. mutans strains studied, and this likely accounts for their enhanced adsorption. Absorption of saliva with 10% octyl or phenyl Sepharose reduced the protein content of saliva by almost half, but the numbers of bacteria which attached to pellicles prepared from such absorbed saliva were similar to or higher than those which attached to control pellicles. These observations suggest that saliva does not contain unique highly hydrophobic salivary macromolecules which serve as essential pellicle receptors for the bacteria studied. The data obtained are consistent with the view that hydrophobic bonding together with interactions between complementary molecules are involved in bacterial attachment to salivary pellicles on teeth.
通过检测口腔细菌吸附十六烷的能力，发现不同口腔细菌的表面疏水性存在差异。黏性放线菌、内氏放线菌、血链球菌、轻链球菌和牙龈拟杆菌菌株表现出高度疏水性。中间普氏菌、唾液链球菌、变形链球菌和产黑色素普氏菌菌株的疏水性较弱，而伴放线放线杆菌菌株具有亲水性。观察到细菌对十六烷的吸附与其附着在羟基磷灰石表面形成的实验性唾液薄膜上的数量之间存在总体相关性。这表明疏水键在这一过程中起重要作用。用氯仿 - 甲醇萃取以去除脂质的唾液制备的薄膜吸附了相当数量的血链球菌和黏性放线菌，以及数量增加的变形链球菌。吸附等温线分析表明，由脱脂唾液形成的薄膜对所研究的变形链球菌菌株含有更多的结合位点，这可能解释了它们吸附增强的原因。用10%辛基或苯基琼脂糖吸附唾液可使唾液蛋白质含量降低近一半，但由这种吸附唾液制备的薄膜上附着的细菌数量与对照薄膜上附着的细菌数量相似或更高。这些观察结果表明，唾液中不含有独特的高度疏水的唾液大分子，这些大分子是所研究细菌的必需薄膜受体。获得的数据与以下观点一致，即疏水键以及互补分子之间的相互作用参与了细菌附着在牙齿上的唾液薄膜的过程。
