Brysk M M, Miller J
J Invest Dermatol. 1983 Sep;81(3):245-8. doi: 10.1111/1523-1747.ep12518246.
Two epidermal cell lines of common origin, one tumorigenic and the other not, have been grown in media of different Ca2+ concentrations (low: 0.07 mM; normal: 1.4 mM; high: 10 mM). At normal Ca2+ levels, both cell lines had similar growth rates. The growth rates were reduced at both higher and lower Ca2+ concentrations, with greater inhibition of the tumorigenic than the nontumorigenic cells. At a given level of Ca2+, the 2 cell lines could not be distinguished in their polyacrylamide gel patterns by protein staining, by [125I]concanavalin A reactivity, and by lactoperoxidase iodination. Changes in surface membrane proteins with Ca2+ concentrations were observed by lactoperoxidase iodination, whereas the other techniques, which measure total cell proteins or glycoproteins, showed no substantial variation.
两种起源相同的表皮细胞系，一种具有致瘤性，另一种无致瘤性，已在不同钙离子浓度（低：0.07 mM；正常：1.4 mM；高：10 mM）的培养基中培养。在正常钙离子水平下，两种细胞系的生长速率相似。在较高和较低钙离子浓度下，生长速率均降低，致瘤性细胞系比非致瘤性细胞系受到的抑制更大。在给定的钙离子水平下，通过蛋白质染色、[125I]伴刀豆球蛋白A反应性和乳过氧化物酶碘化法，无法在聚丙烯酰胺凝胶图谱中区分这两种细胞系。通过乳过氧化物酶碘化法观察到表面膜蛋白随钙离子浓度的变化，而其他测量总细胞蛋白或糖蛋白的技术则未显示出实质性变化。
