人表皮鳞状癌细胞系在不同细胞外钙浓度下的特征

Characteristics of a human epidermal squamous carcinoma cell line at different extracellular calcium concentrations.

作者信息

Brysk M M, Miller J, Walker G K

出版信息

Exp Cell Res. 1984 Feb;150(2):329-37. doi: 10.1016/0014-4827(84)90576-7.

Abstract

A continuous line derived from a human skin squamous cell carcinoma has been grown in media of high, normal and low Ca2+ concentrations. The growth rate was unaffected by the Ca2+ levels even though morphological changes were observed. Desmosomes were absent at low Ca2+ and areas of cell piling were observed at high Ca2+. Cell protein staining patterns on polyacrylamide gels were identical for cells grown at the three Ca2+ levels. The variations were minor for the glycoproteins reacted with 125I-conA. Lactoperoxidase iodination revealed changes in cell surface proteins, most markedly in the emergence of new proteins at high Ca2+.

摘要

源自人皮肤鳞状细胞癌的连续细胞系已在高钙、正常钙和低钙浓度的培养基中培养。尽管观察到形态学变化，但生长速率不受钙水平的影响。在低钙条件下桥粒缺失，在高钙条件下观察到细胞堆积区域。在三种钙水平下生长的细胞在聚丙烯酰胺凝胶上的细胞蛋白质染色模式相同。与125I-伴刀豆球蛋白A反应的糖蛋白变化较小。乳过氧化物酶碘化显示细胞表面蛋白质发生变化，最明显的是在高钙条件下出现新的蛋白质。