Takeda H, Misawa M, Yanaura S
Jpn J Pharmacol. 1983 Apr;33(2):455-61. doi: 10.1254/jjp.33.455.
The effect of bromhexine on behaviors of lysosomal enzymes in the submucosal gland was investigated using canine tracheal slice preparations, with reference to the histochemical changes in acid glycoproteins (AGP) in the gland. Incubation of tracheal slices with 1% Triton X-100 or 0.0004-0.04% bromhexine for 30 min decreased the number of stained lysosomes in the glandular cells. The decrease in stained lysosomes after treatment with 1% Triton X-100 or 0.04% bromhexine was effectively prevented by addition of 5% lecithin. The number of glandular cells that were stained red (stain index R) in the combined alcian blue at pH 2.5 and periodic acid-Schiff procedure markedly increased by treatment with 1% Triton X-100 or 0.0004-0.04% bromhexine. In the bromhexine treated groups, there was a close correlation (r=0.932, P less than 0.01) between the increase in the number of glandular cells showing the stain index R and the decrease in the number of stained lysosomes in the cells. These findings suggest that the enzymes which are liberated from lysosomes into the cytoplasm by bromhexine may, at least in a part, be involved in the mucolytic action of the agent on AGP contained in mucus granules of the submucosal gland.
以犬气管切片标本为材料，参照犬气管黏膜下腺酸性糖蛋白（AGP）的组织化学变化，研究了溴己新对黏膜下腺溶酶体酶活性的影响。用1% Triton X-100或0.0004 - 0.04%溴己新孵育气管切片30分钟，可使腺细胞中染色的溶酶体数量减少。加入5%卵磷脂可有效防止1% Triton X-100或0.04%溴己新处理后染色溶酶体数量的减少。在pH 2.5的阿尔辛蓝和高碘酸-希夫联合染色法中，经1% Triton X-100或0.0004 - 0.04%溴己新处理后，染成红色的腺细胞数量（染色指数R）显著增加。在溴己新处理组中，显示染色指数R的腺细胞数量增加与细胞中染色溶酶体数量减少之间存在密切相关性（r = 0.932，P < 0.01）。这些结果表明，溴己新使溶酶体释放到细胞质中的酶可能至少部分参与了该药物对黏膜下腺黏液颗粒中AGP的黏液溶解作用。
