Takeda H, Kasamatsu S, Misawa M, Yanaura S
Nihon Yakurigaku Zasshi. 1983 Jan;81(1):29-37.
The expectorant effect of brovanexine (BvX) was investigated histologically and histochemically using isolated canine trachea. Following BvX treatment, the number of goblet cells (GC) stained positively with the combination procedure of alcian blue at pH 2.5 or pH 1.0 and periodic acid-Schiff was unaffected. The number of GC which stained blue and purple (stain index B & P) was reduced, while the number of GC that stained red (stain index R) was increased. The thickness of the acini of submucosal glands (SG) markedly decreased with 10(-5) and 10(-4)M BvX, and the ratio of the acinar inner diameter to the tracheal wall thickness significantly increased with 10(-4)M BvX. Stain index B & P of glandular cells decreased with 10(-5) and 10(-4)M BvX, which was accompanied by a marked increase in the stain index R. BvX treatment caused increases in total saccharide and protein concentrations in the incubation fluid, while N-acetylhexosamine slightly decreased. Both the BvX-induced histological changes in glandular cells and changes in concentrations of macromolecular components in the incubation fluid were much the same degree as those induced by bromhexine (Bh). The BvX-induced histochemical changes in secretory cells, however, were slighter than those induced by Bh. These findings suggest that BvX is an expectorant possessing both the secretagogic action selectively on SG and a mucolytic action toward acid glycoproteins in granules of secretory cells.
使用离体犬气管，通过组织学和组织化学方法研究了溴凡克新（BvX）的祛痰作用。BvX处理后，用pH 2.5或pH 1.0的阿尔辛蓝与过碘酸希夫氏联合染色法呈阳性染色的杯状细胞（GC）数量未受影响。染成蓝色和紫色的GC数量（染色指数B&P）减少，而染成红色的GC数量（染色指数R）增加。10(-5)和10(-4)M的BvX可使黏膜下腺（SG）腺泡厚度显著降低，10(-4)M的BvX可使腺泡内径与气管壁厚度之比显著增加。10(-5)和10(-4)M的BvX可使腺细胞的染色指数B&P降低，同时染色指数R显著增加。BvX处理可使孵育液中总糖和蛋白质浓度增加，而N-乙酰己糖胺略有降低。BvX诱导的腺细胞组织学变化以及孵育液中大分子成分浓度的变化与溴己新（Bh）诱导的变化程度大致相同。然而，BvX诱导的分泌细胞组织化学变化比Bh诱导的变化轻微。这些发现表明，BvX是一种祛痰剂，对SG具有选择性促分泌作用，并对分泌细胞颗粒中的酸性糖蛋白具有黏液溶解作用。
