Yanaura S, Takeda H, Nishimura T, Misawa M
Nihon Yakurigaku Zasshi. 1981 Jul;78(1):17-25.
The effects of bromhexine on secretory activities of canine tracheal secretory cells with stimulated synthesis of acid glycoprotein (AGP) by pilocarpine 10(-6)M treatment an on behaviour of glycoprotein in these secretory cells were investigated histologically and histochemically. Following bromhexine treatment, the number of total glycoprotein-containing goblet cells (GC) remained unchanged. The numbers of AGP-containing and sulphated glycoprotein (SGP)-containing GC significantly decreased, while neutral glycoprotein (NGP)-containing GC significantly increased. The acinar inner diameter of the submucosal gland and the acinar inner diameter of this gland to wall ratio slightly increased. Thickness of the acinus and Reid index slightly decreased, concentration-dependently. AGP and SGP content in glandular cells decreased, while NGP content in these cells markedly increased. Bromhexine 10(-5) and 10(-4)M treatment resulted in increased total saccharide and protein concentrations in the incubation fluid, whereas the agent significantly decreased N-acetylhexosamine, in a concentration-dependent manner. These findings suggest that while bromhexine does not stimulate secretory activities of GC, it does slightly stimulate the activities of the submucosal glands. Bromhexine markedly dissolves AGP in the granules of these secretory cells.
研究了溴己新对犬气管分泌细胞分泌活动的影响，这些细胞经10(-6)M毛果芸香碱处理后酸性糖蛋白（AGP）合成受到刺激，同时还研究了溴己新对这些分泌细胞中糖蛋白行为的影响，采用了组织学和组织化学方法。溴己新处理后，含总糖蛋白的杯状细胞（GC）数量保持不变。含AGP和含硫酸化糖蛋白（SGP）的GC数量显著减少，而含中性糖蛋白（NGP）的GC数量显著增加。黏膜下腺的腺泡内径及其与腺泡壁厚度的比值略有增加。腺泡厚度和Reid指数略有下降，呈浓度依赖性。腺细胞中AGP和SGP含量下降，而这些细胞中NGP含量显著增加。10(-5)和10(-4)M的溴己新处理导致孵育液中总糖和蛋白质浓度增加，而该药物以浓度依赖性方式显著降低N-乙酰己糖胺。这些发现表明，虽然溴己新不刺激GC的分泌活动，但它确实轻微刺激黏膜下腺的活动。溴己新能显著溶解这些分泌细胞颗粒中的AGP。
