在革兰氏阴性细菌木醋杆菌的包膜中，与纤维素产生相关的孔（输出位点）的可视化。
Visualization of pores (export sites) correlated with cellulose production in the envelope of the gram-negative bacterium Acetobacter xylinum.
作者信息
Zaar K
出版信息
J Cell Biol. 1979 Mar;80(3):773-7. doi: 10.1083/jcb.80.3.773.
Abstract
The Gram-negative bacterium Acetobacter xylinum assembles a cellulse ribbon composed of a number of microfibrils in the longitudinal axis of its envelope. The zone of ribbon assembly was investigated by freeze-etch electron microscopy. Freeze-etching revealed, beneath the cellulose ribbons, a linear array of pores on the lipopolysaccharide membrane. These pores have a rim diameter of 120--150 A and a central hole or deepening of approximately 35 A. The axes of pore arrays closely coincide with linear arrays of 100 A particles on the E- and P-faces of the fractured lipopolysaccharide membranes. Pores and particles in the lipopolysaccharide membrane are probably congruent. The pores are hypothesized to be the export sites (penetration sites) for cellulose.
摘要
革兰氏阴性菌木醋杆菌在其包膜的纵轴上组装由许多微纤维组成的纤维素带。通过冷冻蚀刻电子显微镜研究了纤维素带的组装区域。冷冻蚀刻显示，在纤维素带下方，脂多糖膜上有一排线性排列的孔。这些孔的边缘直径为120 - 150埃，中心孔或凹陷约为35埃。孔阵列的轴与破裂的脂多糖膜的E面和P面上100埃颗粒的线性阵列紧密重合。脂多糖膜中的孔和颗粒可能是一致的。推测这些孔是纤维素的输出位点（穿透位点）。
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