Rajguru S U, Kang Y H, Ahluwalia B S
J Nutr. 1982 Oct;112(10):1881-91. doi: 10.1093/jn/112.10.1881.
The purpose of the study was to determine the intracellular location of exogenously injected labeled vitamin A (retinol) in rat testes by light and electron microscopic autoradiography. Tritium-labeled vitamin A mixed in vitamin A-free rat serum was injected intracapsularly in one testis in adult rats. At intervals of 30 minutes, 2 hours, and 4 hours following injection the treated testes were removed the processed for autoradiography. The results show that in the testes vitamin A is localized at three sites: within the macrophages of the interstitial tissue, in the lipid droplets of the Sertoli cells, and in the spermatids in association with Golgi saccules. To confirm the autoradiographic localization of vitamin A, the cellular retinol-binding protein (CRBP) assay demonstrates the presence of CRBP in the interstitial tissue and seminiferous tubules. The results of the study suggest a possible role of vitamin A in spermiogenesis in rat testes.
本研究的目的是通过光学显微镜和电子显微镜放射自显影术确定外源性注射的标记维生素A（视黄醇）在大鼠睾丸中的细胞内定位。将氚标记的维生素A与不含维生素A的大鼠血清混合，成年大鼠一侧睾丸经囊内注射。注射后30分钟、2小时和4小时，分别取出处理过的睾丸进行放射自显影术处理。结果显示，在睾丸中维生素A定位于三个部位：间质组织的巨噬细胞内、支持细胞的脂滴中以及与高尔基体泡相关的精子细胞中。为了证实维生素A的放射自显影定位，细胞视黄醇结合蛋白（CRBP）检测表明在间质组织和生精小管中存在CRBP。该研究结果提示维生素A在大鼠睾丸精子发生过程中可能发挥作用。
