四种卵菌纲和壶菌纲水生霉菌5S核糖体RNA的核苷酸序列。
Nucleotide sequences of 5S ribosomal RNA from four oomycete and chytrid water molds.
作者信息
Walker W F, Doolittle W F
出版信息
Nucleic Acids Res. 1982 Sep 25;10(18):5717-21. doi: 10.1093/nar/10.18.5717.
Abstract
The nucleotide sequences of the 5S rRNAs of the oomycete water molds Saprolegnia ferax and Pythium hydnosporum and of the chytrid water molds Blastocladiella simplex and Phlyctochytrium irregulare were determined by chemical and enzymatic partial degradation of 3' and 5' end-labelled molecules, followed by gel sequence analysis. The two oomycete sequences differed in 24 positions and the two chytrid sequences differed in 27 positions. These pairs differed in a mean of 44 positions. The chytrid sequences clearly most resemble the sequence from the zygomycete Phycomyces, while the oomycete sequences appear to be allied with those from protozoa and slime molds.
摘要
通过对3'和5'末端标记分子进行化学和酶促部分降解，随后进行凝胶序列分析，确定了卵菌水霉（绵霉）和腐霉以及壶菌水霉（简单芽枝霉）和不规则根壶菌的5S rRNA的核苷酸序列。两种卵菌序列在24个位置上不同，两种壶菌序列在27个位置上不同。这两对序列平均相差44个位置。壶菌序列显然与接合菌毛霉的序列最为相似，而卵菌序列似乎与原生动物和黏菌的序列相关。
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