Corvalán J R, Howard J C
Eur J Immunol. 1978 May;8(5):331-5. doi: 10.1002/eji.1830080508.
Rat spleen cells are shown to be unresponsive to sheep red cells (SRBC) in vitro under conditions in which thoracic duct lymphocytes (TDL) respond very well. By adding unresponsive spleen cells to responsive TDL cultures, the spleen cells are shown to contain an inhibitor capable of preventing the response to SRBC. The inhibitory activity is a property of live cells; it is sensitive to radiation doses as low as 100 R x rays and to mitomycin C. It can be completely removed from spleen cell suspensions by extraction with large amounts of carbonyl iron or by filtration through nylon wool columns. It is less efficiently removed by filtration through Sephades G-10 columns, and is completely resistant to the cytotoxic effects of silica. From a practical point of view, extraction of a spleen cell suspension with carbonyl iron is a useful method of obtaining fully responsive lymphocyte populations from rat spleen.
在胸导管淋巴细胞（TDL）对绵羊红细胞（SRBC）反应良好的体外条件下，大鼠脾细胞对SRBC无反应。通过将无反应的脾细胞添加到有反应的TDL培养物中，发现脾细胞含有一种能够阻止对SRBC反应的抑制剂。这种抑制活性是活细胞的一种特性；它对低至100拉德X射线的辐射剂量和丝裂霉素C敏感。通过用大量羰基铁提取或通过尼龙棉柱过滤，可以从脾细胞悬液中完全去除它。通过Sephades G - 10柱过滤去除的效率较低，并且它对二氧化硅的细胞毒性作用完全有抗性。从实际角度来看，用羰基铁提取脾细胞悬液是从大鼠脾脏获得完全有反应的淋巴细胞群体的一种有用方法。
