Toorop A I, Gribling-Hegge L, Meijs-Roelofs H M
J Endocrinol. 1982 Dec;95(3):287-92. doi: 10.1677/joe.0.0950287.
The present study examined the ovarian progesterone levels in rats during the normal 5-day oestrous cycle and in rats with an experimentally induced delayed or advanced ovulation. Ovarian androgen and oestrogen concentrations were also studied in rats with an advanced ovulation. The ovarian progesterone concentrations during the normal cycle showed two peaks: one during metoestrus and dioestrus 1 and the second during the afternoon of pro-oestrus. Rats treated with pentobarbitone sodium (35 mg/kg body weight) at 13.00 h on the day of pro-oestrus showed no increase in ovarian progesterone levels on that day, but did show an increase during the afternoon of the next day. Rats injected intraperitoneally with an ovulation-inducing bolus of human chorionic gonadotrophin (hCG; 20 i.u.) at 15.00 h on the day before pro-oestrus (dioestrus 2) showed increased ovarian progesterone levels as early as 0.5 h after hCG injection. Lowered levels of ovarian androgen and oestrogen values compared with saline-injected rats were first found 2 and 6 h respectively after hCG injection. In conjunction with data obtained previously these findings show that normal, delayed and advanced ovulations are preceded by an increase in ovarian progesterone concentrations and a subsequent decline in ovarian androgen and oestrogen levels.
本研究检测了正常5天发情周期大鼠以及实验诱导排卵延迟或提前的大鼠的卵巢孕酮水平。同时也研究了排卵提前的大鼠的卵巢雄激素和雌激素浓度。正常周期中卵巢孕酮浓度出现两个峰值：一个在间情期和动情间期1，另一个在发情前期下午。发情前期当天13:00用戊巴比妥钠（35mg/kg体重）处理的大鼠，当天卵巢孕酮水平未升高，但在次日下午出现升高。发情前期前一天（动情间期2）15:00腹腔注射人绒毛膜促性腺激素（hCG；20国际单位）促排卵大剂量的大鼠，在hCG注射后0.5小时卵巢孕酮水平就开始升高。与注射生理盐水的大鼠相比，hCG注射后分别在2小时和6小时首次发现卵巢雄激素和雌激素水平降低。结合之前获得的数据，这些发现表明正常、延迟和提前排卵之前卵巢孕酮浓度会升高，随后卵巢雄激素和雌激素水平会下降。
