采用包括柱后衍生化和荧光检测的高效液相色谱法分析人血浆中的6-巯基嘌呤。

Analysis of 6-mercaptopurine in human plasma with a high-performance liquid chromatographic method including post-column derivatization and fluorimetric detection.

作者信息

Jonkers R E, Oosterhuis B, ten Berge R J, van Boxtel C J

出版信息

J Chromatogr. 1982 Dec 10;233:249-55. doi: 10.1016/s0378-4347(00)81751-x.

Abstract

A relatively simple assay with improved reliability and sensitivity for measuring levels of 6-mercaptopurine in human plasma is presented. After extraction of the compound and the added internal standard with phenyl mercury acetate, samples were separated by ion-pair reversed-phase high-performance liquid chromatography. On-line the analytes were oxidized to fluorescent products and detected in a flow-fluorimeter. The within-day coefficient of variation was 3.8% at a concentration of 25 ng/ml. The lower detection limit was 2 ng/ml when 1.0 ml of plasma was used. Mercaptopurine concentration versus time curves of two subjects after a single oral dose of azathioprine are shown.

摘要

本文介绍了一种相对简单的测定方法，用于测量人血浆中6-巯基嘌呤的水平，该方法具有更高的可靠性和灵敏度。用醋酸苯汞萃取化合物和添加的内标后，样品通过离子对反相高效液相色谱进行分离。在线将分析物氧化为荧光产物，并在流动荧光计中进行检测。在浓度为25 ng/ml时，日内变异系数为3.8%。当使用1.0 ml血浆时，检测下限为2 ng/ml。展示了两名受试者单次口服硫唑嘌呤后巯基嘌呤浓度随时间的曲线。