使用高效液相色谱法定量生物流体中的6-巯基嘌呤：一种选择性的新方法。

Quantitation of 6-mercaptopurine in biologic fluids using high-performance liquid chromatography: a selective and novel procedure.

作者信息

Narang P K, Yeager R L, Chatterji D C

出版信息

J Chromatogr. 1982 Jul 9;230(2):373-80. doi: 10.1016/s0378-4347(00)80487-9.

PMID:7202015

Abstract

A simple, selective, sensitive and rapid procedure is described for the quantitation of 6-mercaptopurine (6-MP) in biological fluids. A sensitivity of at least 5 ng/ml is easily achieved in plasma on a reversed-phase octadecylsilane (C18) column using a high-performance liquid chromatography system following an initial protein precipitation and a clean-up step. Mean extractability of the drug from plasma following this procedure is greater than 98% and the overall coefficient of variation for the assay is below 6%. Plasma levels of 6-MP were measured in a rhesus monkey for 12 h following an intravenous administration of a single bolus dose (4 mg/kg) of 6-MP.

摘要

本文描述了一种用于定量生物体液中6-巯基嘌呤（6-MP）的简单、选择性、灵敏且快速的方法。使用高效液相色谱系统，在初始蛋白质沉淀和净化步骤之后，于反相十八烷基硅烷（C18）柱上，血浆中轻松实现至少5 ng/ml的灵敏度。按照此方法，药物从血浆中的平均提取率大于98%，该测定的总体变异系数低于6%。在恒河猴静脉注射单次推注剂量（4 mg/kg）的6-MP后12小时内测定其血浆中6-MP水平。