Qureshi A A, Burger W C, Elson C E, Benevenga N J
Lipids. 1982 Dec;17(12):924-34. doi: 10.1007/BF02534588.
Swine were fed corn- or barley-based diets with, or without, culture filtrate (CF) of Trichoderma viride for 21 days. Weight gains were nonsignificantly but slightly increased by CF. The activities of beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7 alpha-hydroxylase, acetyl-CoA carboxylase (ACX), fatty acid synthetase (FAS) and other lipogenic enzymes in several tissues were determined. Significant decreases in the activities of HMG-CoA reductase and cholesterol 7 alpha-hydroxylase in all tissues of swine fed the CF-diets were observed. The major site for the regulation of cholesterol biosynthesis was adipose tissue followed by the intestine, liver, lung and muscle in order of activity. The concentrations of cholesterol in serum and muscle were decreased 27% and 23%, respectively, by CF. ACX and FAS activities increased ca. 2-fold when CF was fed with either of the cereal-based diets. The major sites for fatty acid synthesis was the adipose tissue and, to a lesser extent, the liver. Very low rates of synthesis were detected in intestine, lung and muscle. Similar distributions of activities were found for related lipogenic enzymes.
给猪喂食以玉米或大麦为基础的日粮，添加或不添加绿色木霉培养滤液（CF），持续21天。CF使猪的体重增加不显著但略有增加。测定了几种组织中β-羟基-β-甲基戊二酰辅酶A（HMG-CoA）还原酶、胆固醇7α-羟化酶、乙酰辅酶A羧化酶（ACX）、脂肪酸合成酶（FAS）和其他脂肪生成酶的活性。观察到喂食含CF日粮的猪所有组织中HMG-CoA还原酶和胆固醇7α-羟化酶的活性显著降低。胆固醇生物合成的主要调节部位是脂肪组织，其次是肠道、肝脏、肺和肌肉，按活性顺序排列。CF使血清和肌肉中的胆固醇浓度分别降低了27%和23%。当CF与任何一种谷物日粮一起喂食时，ACX和FAS的活性增加了约2倍。脂肪酸合成的主要部位是脂肪组织，其次是肝脏。在肠道、肺和肌肉中检测到的合成速率非常低。相关脂肪生成酶的活性分布相似。
