Wyroba E
Cytobiologie. 1978 Aug;17(2):412-20.
Treatment of Paramecium aurelia with trypsin or pronase (1 mg per 10(5) cells, at 0 to 4 degrees C) partially removes the surface coat and modifies significantly multiplication of cells. The division rate after 24 hours of cultivation is diminished approximately twice in the case of pronase-treated cells and 1.5 for tyrpsin-digested ciliates as compared with the control. On the second day the division rate increases rapidly and number of cell divisions exceeds the values observed in the control. After 72 hours of cultivation the division rate in both untreated and enzyme-treated cells is almost the same. It is concluded that the observed inhibition of cell fission results from the enzymatic removal of the surface coat--the integrity of this surface coat seems to be necessary in the process of cell division. The influence of environmental factors on the rate of growth is presented.
用胰蛋白酶或链霉蛋白酶（每10⁵个细胞1毫克，在0至4摄氏度下）处理草履虫会部分去除其表面被膜，并显著改变细胞增殖。与对照相比，经链霉蛋白酶处理的细胞培养24小时后的分裂率大约降低两倍，经胰蛋白酶消化的纤毛虫降低1.5倍。第二天分裂率迅速增加，细胞分裂数超过对照中观察到的值。培养72小时后，未处理和经酶处理的细胞的分裂率几乎相同。得出的结论是，观察到的细胞分裂抑制是由于表面被膜的酶促去除——这种表面被膜的完整性在细胞分裂过程中似乎是必要的。还介绍了环境因素对生长速率的影响。
