Kanjanapone V, Hartig-Beecken I, Epstein M F
Pediatr Res. 1980 Apr;14(4 Pt 1):278-81. doi: 10.1203/00006450-198004000-00003.
To examine the effect of beta adrenergic drugs on fetal lung development, we administered isoxsuprine to pregnant rabbits for 24 hr and measured indices of pulmonary surfactant synthesis, storage, and release in rabbit fetuses at 26 days gestation. Incorporation of radiolabeled choline into total and disaturated phosphatidylcholine was measured in vitro in fetal lung slices. There was a significant increase in the rate of choline incorporation into disaturated phosphatidylcholine in the isoxsuprine-treated group and a tendency toward an increased incorporation into total phosphatidylcholine as well. We also observed an increase in the pulmonary phospholipid reservoir as evidenced by a significant increase in total lung disaturated phosphatidylcholine and a trend toward higher total lung phosphatidylcholine in the isoxsuprine group. In addition, there was a significant increase in lung lavage L/S ratio in the treated fetuses and in lung deflation stability determined by pressure volume curve. We conclude that isoxsuprine increases synthesis, storage, and release of surfactant in rabbit fetuses at 26 days gestation.
为研究β肾上腺素能药物对胎儿肺发育的影响，我们给怀孕的兔子注射异舒普林24小时，并在妊娠26天时测量兔胎儿肺表面活性物质合成、储存和释放的指标。在体外测量胎儿肺切片中放射性标记胆碱掺入总磷脂酰胆碱和二饱和磷脂酰胆碱的情况。在异舒普林治疗组中，胆碱掺入二饱和磷脂酰胆碱的速率显著增加，并且掺入总磷脂酰胆碱也有增加的趋势。我们还观察到肺磷脂储备增加，异舒普林组肺总二饱和磷脂酰胆碱显著增加以及肺总磷脂酰胆碱有升高趋势证明了这一点。此外，治疗胎儿的肺灌洗L/S比值以及通过压力-容积曲线测定的肺萎陷稳定性均显著增加。我们得出结论，异舒普林可增加妊娠26天兔胎儿肺表面活性物质的合成、储存和释放。
