Corbet A J, Flax P, Rudolph A J
J Appl Physiol Respir Environ Exerc Physiol. 1977 Dec;43(6):1039-45. doi: 10.1152/jappl.1977.43.6.1039.
After the maternal abdomen was opened under methoxyflurane anesthesia, fetal rabbits of 27.5 days gestation were given injections through the intact uterine wall of saline, pilocarpine, isoxsuprine, muscarine, phenylephrine, atropine, phenoxybenzamine, or propranolo, alone or in appropriate combinations. Fetal rabbits were delivered by hysterotomy and killed without breathing 2.5 h later. Static pressure-volume curves with air showed improved retention on deflation in fetal rabbits that had injections of pilocarpine, or isoxsuprine, but not of muscarine or phenylephrine. The effect of pilocarpine on the pressure-volume curve was blocked by atropine, phenoxybenzamine, and propranolol, and the effect of isoxsuprine was blocked by propranolol but not phenoxybenzamine. The data suggest that pilocarpine produces secretion of surfactant into lung air spaces by exciting the sympathetic nervous system, a known function of pilocarpine, rather than the parasympathetic nervous system. This may result in stimulation of the same beta-adrenergic receptors affected by isoxsuprine which is also thought to stimulate surfactant secretion.
在甲氧氟烷麻醉下打开母兔腹部后，对妊娠27.5天的胎兔通过完整的子宫壁注射生理盐水、毛果芸香碱、异舒普林、毒蕈碱、去氧肾上腺素、阿托品、酚苄明或普萘洛尔，单独注射或进行适当组合。通过剖腹取胎娩出胎兔，2.5小时后在其未呼吸时将其处死。对注射过毛果芸香碱或异舒普林（而非毒蕈碱或去氧肾上腺素）的胎兔进行的空气静态压力-容积曲线显示，放气时肺容量保留情况有所改善。毛果芸香碱对压力-容积曲线的作用可被阿托品、酚苄明和普萘洛尔阻断，而异舒普林的作用可被普萘洛尔阻断，但不能被酚苄明阻断。数据表明，毛果芸香碱是通过兴奋交感神经系统而非副交感神经系统，促使表面活性物质分泌到肺腔中，这是毛果芸香碱的一个已知功能。这可能导致对与异舒普林作用的相同β-肾上腺素能受体的刺激，而异舒普林也被认为可刺激表面活性物质分泌。
