Fotsis T, Järvenpää P, Adlercreutz H
J Clin Endocrinol Metab. 1980 Jul;51(1):148-51. doi: 10.1210/jcem-51-1-148.
After enzymatic hydrolysis and various chromatographic steps, an estrogen metabolite behaving like 4-hydroxyestriol in capillary gas chromatography on several stationary phases was detected in pregnancy urine. The mass spectrum of its trimethylsilyl ether derivative showed the very characteristic fragmentation pattern of catechol estrogens and was found to be practically identical with that of the corresponding derivative of the reference standard. In addition, selected ion monitoring evidence of the identity of the new estrogen metabolite with 4-hydroxyestriol was obtained. It is concluded that the results confirm previous studies indicating formation of 4-hydroxylated estrogen metabolites in human tissues.
经过酶水解和各种色谱步骤后，在孕妇尿液中检测到一种雌激素代谢物，在几种固定相上的毛细管气相色谱中其行为类似于4-羟基雌三醇。其三甲基硅醚衍生物的质谱显示出儿茶酚雌激素非常特征性的碎片模式，并且发现与参考标准品的相应衍生物几乎相同。此外，还获得了新的雌激素代谢物与4-羟基雌三醇身份相同的选择离子监测证据。结论是，这些结果证实了先前的研究，表明人体组织中形成了4-羟基化雌激素代谢物。
