Lindgren K
Clin Chem. 1986 Jul;32(7):1397-9.
I describe a chromatographic procedure for quantification of urinary estriol in late pregnancy, with 6 alpha-hydroxyestriol used as internal standard. The hydrolyzed sample is successively passed through a Bond Elut C18 column and a DEAE-Sephadex column. The estrogens are then completely resolved as silyl ethers on a packed gas-liquid chromatographic (GLC) column in isothermal mode. Analytical recoveries of conjugated estriol ranged between 96 and 102%. Between-day precision (CV) was 4.4% at a mean concentration of 85.1 mumol/L. Specificity was assessed by comparing results obtained with a capillary GLC column.
我描述了一种用于定量妊娠晚期尿中雌三醇的色谱方法，使用6α-羟基雌三醇作为内标。水解后的样品依次通过Bond Elut C18柱和DEAE-葡聚糖凝胶柱。然后，雌激素在填充气液色谱（GLC）柱上以等温模式完全分离为硅烷醚。结合型雌三醇的分析回收率在96%至102%之间。在平均浓度为85.1μmol/L时，日间精密度（CV）为4.4%。通过比较毛细管GLC柱获得的结果来评估特异性。
