阿米巴样运动的收缩基础。VII. 荧光标记肌动蛋白在活阿米巴中 的分布。
Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.
作者信息
Taylor D L, Wang Y L, Heiple J M
出版信息
J Cell Biol. 1980 Aug;86(2):590-8. doi: 10.1083/jcb.86.2.590.
Abstract
The technique of molecular cytochemistry has been used to follow the distribution of fluorescently labeled actin in living Chaos carolinensis and Amoeba proteus during ameboid movement and various cellular processes. The distribution of 5-iodoacetamidofluorescein-labeled actin was compared with that of Lissamine rhodamine B sulfonyl chloride-labeled ovalbumin microinjected into the same cell and recorded with an image intensification microscope system. Actively motile cells demonstrated a rather uniform distribution of actin throughout most of the cytoplasm, except in the tail ectoplasm and in plasma gel sheets, where distinct actin structures were observed. In addition, actin-containing structures were induced in the cortex during wound healing, concanavalin A capping, pinocytosis, and contractions elicited by phalloidin injections. The formation of distinct fluorescent actin structures has been correlated with contractile activities.
摘要
分子细胞化学技术已被用于追踪荧光标记的肌动蛋白在活的卡罗林游仆虫和变形虫进行阿米巴运动及各种细胞过程时的分布情况。将5-碘乙酰氨基荧光素标记的肌动蛋白的分布与注射到同一细胞中的丽丝胺罗丹明B磺酰氯标记的卵清蛋白的分布进行比较，并用图像增强显微镜系统进行记录。活跃运动的细胞在大部分细胞质中呈现出相当均匀的肌动蛋白分布，但在尾部外质和质膜凝胶片中除外，在这些部位观察到了明显的肌动蛋白结构。此外，在伤口愈合、伴刀豆球蛋白A帽化、胞饮作用以及由鬼笔环肽注射引发的收缩过程中，皮质中会诱导出含肌动蛋白的结构。不同荧光肌动蛋白结构的形成与收缩活动相关。
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