关于人细胞中一种损伤特异性DNA结合蛋白的底物特异性
On the substrate specificity of a damage-specific DNA binding protein from human cells.
作者信息
Feldberg R S
出版信息
Nucleic Acids Res. 1980 Mar 11;8(5):1133-43. doi: 10.1093/nar/8.5.1133.
Abstract
The nature of the lesion recognized by a damage-specific DNA binding protein from human cells was investigated by examining the substrate specificity of this protein. Protein-recognizable damage was introduced into both T7 DNA and Poly d(A-T) at relatively low UV fluences. In addition, the protein demonstrated binding to both nitrous acid- and bisulfite-treated DNA, but not to DNA crosslinked with trioxsalen plus near-UV nor to non-irradiated uracil-containing DNA. These results suggest that this protein could be recognizing minor helix distortions in DNA rather than any one single lesion.
摘要
通过检测一种来自人类细胞的损伤特异性DNA结合蛋白的底物特异性，对该蛋白所识别的损伤性质进行了研究。在相对较低的紫外线通量下，将蛋白质可识别的损伤引入T7 DNA和聚d(A-T)中。此外，该蛋白显示出与亚硝酸和亚硫酸氢盐处理的DNA结合，但不与三氧沙林加近紫外线交联的DNA或未辐照的含尿嘧啶DNA结合。这些结果表明，该蛋白可能识别的是DNA中的微小螺旋扭曲，而非任何一种单一损伤。
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