Suppr超能文献

一种DNA结合蛋白对化学致癌物修饰的DNA的识别。

Recognition of chemical carcinogen-modified DNA by a DNA-binding protein.

作者信息

Moranelli F, Lieberman M W

出版信息

Proc Natl Acad Sci U S A. 1980 Jun;77(6):3201-5. doi: 10.1073/pnas.77.6.3201.

DOI:10.1073/pnas.77.6.3201
PMID:6932015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC349582/
Abstract

Using a filter binding assay, we have detected and partially purified a protein from human placenta that has a high affinity for N-acetoxy-2-acetylaminofluorene-modified double-stranded DNA (AAF-[3H]DNA) of bacteriophage T7. This protein has been partially purified from a 1 M NaCl extract of a crude nuclear fraction by a combination of ion-exchange and nucleic acid affinity chromatography. With AAF-[3H]DNA as the substrate, the binding reaction reached equlibrium within 1 hr at 4 degrees C, and the extent of binding ws proportional to the amount of protein added. Complex formation was dependent on both pH and salt concentration and was unaffected by the presence of sulfhydryl-blocking agents. The purest protein fraction also recognizes DNA modified with methylmethane-sulfonate or methylnitrosourea. It shows little or no recognition of single-stranded DNA, double-stranded DNA, supercoiled bacteriophage phiX174 DNA, partially depurinated DNA, glucosylated bacteriophage T4DNA, or UV-irradiated DNA. No endo- or exonuclease activity, DNA polymerase activity, or glucosylase activity for AAF-DNA was detectable in the preparation.

摘要

我们使用滤膜结合试验，从人胎盘中检测并部分纯化了一种蛋白质，该蛋白质对噬菌体T7的N-乙酰氧基-2-乙酰氨基芴修饰的双链DNA（AAF-[3H]DNA）具有高亲和力。通过离子交换和核酸亲和色谱相结合的方法，已从粗核级分的1 M NaCl提取物中部分纯化了该蛋白质。以AAF-[3H]DNA为底物，结合反应在4℃下1小时内达到平衡，结合程度与添加的蛋白质量成正比。复合物的形成取决于pH值和盐浓度，不受巯基封闭剂的影响。最纯的蛋白质组分也能识别经甲基磺酸甲酯或甲基亚硝基脲修饰的DNA。它对单链DNA、双链DNA、超螺旋噬菌体phiX174 DNA、部分脱嘌呤的DNA、糖基化噬菌体T4 DNA或紫外线照射的DNA几乎没有或没有识别能力。在制备物中未检测到针对AAF-DNA的内切或外切核酸酶活性、DNA聚合酶活性或糖基化酶活性。

相似文献

1
Recognition of chemical carcinogen-modified DNA by a DNA-binding protein.一种DNA结合蛋白对化学致癌物修饰的DNA的识别。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3201-5. doi: 10.1073/pnas.77.6.3201.
2
DNA binding spectrum of the carcinogen N-acetoxy-N-2-acetylaminofluorene significantly differs from the mutation spectrum.致癌物N-乙酰氧基-N-2-乙酰氨基芴的DNA结合谱与突变谱显著不同。
J Mol Biol. 1984 Jul 25;177(1):173-80. doi: 10.1016/0022-2836(84)90063-9.
3
DNA-binding protein from HeLa cells that binds preferentially to supercoiled DNA damaged by ultraviolet light or N-acetoxy-N-acetyl-2-aminofluorene.来自HeLa细胞的DNA结合蛋白，它优先结合被紫外线或N-乙酰氧基-N-乙酰-2-氨基芴损伤的超螺旋DNA。
Biochim Biophys Acta. 1982 May 31;697(2):202-12. doi: 10.1016/0167-4781(82)90078-1.
4
DNA excision-repair deficiency of human peripheral blood lymphocytes treated with chemical carcinogens.
Cancer Res. 1976 Apr;36(4):1397-403.
5
Enzymatic methylation of chicken erythrocyte DNA modified by two carcinogens, 2-(N-acetoxyacetylamino) fluorene and methylnitrosourea.两种致癌物2-（N-乙酰氧基乙酰氨基）芴和甲基亚硝基脲修饰的鸡红细胞DNA的酶促甲基化作用
Recent Results Cancer Res. 1983;84:193-201. doi: 10.1007/978-3-642-81947-6_14.
6
Reevaluation of a DNA binding activity with specificity for chemically modified DNA.
Carcinogenesis. 1982;3(6):719-21. doi: 10.1093/carcin/3.6.719.
7
Reactivity of antibodies to guanosine modified by the carcinogen N-acetoxy-N-2-acetylaminofluorene.抗体对由致癌物N-乙酰氧基-N-2-乙酰氨基芴修饰的鸟苷的反应性。
Nucleic Acids Res. 1979 Feb;6(2):733-44. doi: 10.1093/nar/6.2.733.
8
Evidence that N-acetoxy-N-acetyl-2-aminofluorene crosslinks DNA to protein by a free radical mechanism.
Carcinogenesis. 1983;4(5):501-7. doi: 10.1093/carcin/4.5.501.
9
High blood pressure related to carcinogen-induced unscheduled DNA synthesis, DNA carcinogen binding, and chromosomal aberrations in human lymphocytes.高血压与致癌物诱导的人类淋巴细胞非程序性DNA合成、DNA致癌物结合及染色体畸变有关。
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2496-500. doi: 10.1073/pnas.73.7.2496.
10
Purification of DNA-binding proteins of bacteriophage T3 and heir role in in vitro packaging of phage T3 DNA.噬菌体T3 DNA结合蛋白的纯化及其在噬菌体T3 DNA体外包装中的作用。
Virology. 1980 Sep;105(2):480-9. doi: 10.1016/0042-6822(80)90048-3.

引用本文的文献

1
Evidence for a novel DNA damage binding protein in human cells.人类细胞中一种新型DNA损伤结合蛋白的证据。
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6918-23. doi: 10.1073/pnas.93.14.6918.
2
A damage-recognition protein which binds to DNA containing interstrand cross-links is absent or defective in Fanconi anemia, complementation group A, cells.在范可尼贫血互补组A细胞中，一种与含有链间交联的DNA结合的损伤识别蛋白缺失或存在缺陷。
Nucleic Acids Res. 1993 Sep 11;21(18):4187-92. doi: 10.1093/nar/21.18.4187.
3
DNA-binding proteins of human placenta: purification and characterization of an endonuclease.人胎盘的DNA结合蛋白：一种核酸内切酶的纯化与特性分析
Mol Biol Rep. 1984 Dec;10(2):91-7. doi: 10.1007/BF00776980.
4
A low polymorphic mouse H-2 class I gene from the Tla complex is expressed in a broad variety of cell types.来自Tla复合体的一个低多态性小鼠H-2 I类基因在多种细胞类型中表达。
J Exp Med. 1987 Aug 1;166(2):341-61. doi: 10.1084/jem.166.2.341.
5
Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP.酵母RAD3蛋白中赖氨酸-48突变为精氨酸会消除其ATP酶和DNA解旋酶活性，但不会消除其结合ATP的能力。
EMBO J. 1988 Oct;7(10):3263-9. doi: 10.1002/j.1460-2075.1988.tb03193.x.
6
Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II).
Proc Natl Acad Sci U S A. 1989 Nov;86(21):8328-32. doi: 10.1073/pnas.86.21.8328.
7
A constitutive damage-specific DNA-binding protein is synthesized at higher levels in UV-irradiated primate cells.一种组成型损伤特异性DNA结合蛋白在紫外线照射的灵长类细胞中合成水平更高。
Mol Cell Biol. 1990 May;10(5):2041-8. doi: 10.1128/mcb.10.5.2041-2048.1990.
8
Human cells contain protein specifically binding to a single 1,N6-ethenoadenine in a DNA fragment.
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6839-42. doi: 10.1073/pnas.88.15.6839.
9
An ultraviolet light-damaged DNA recognition protein absent in xeroderma pigmentosum group E cells binds selectively to pyrimidine (6-4) pyrimidone photoproducts.一种在着色性干皮病E组细胞中缺失的紫外线损伤DNA识别蛋白可选择性地结合嘧啶（6-4）嘧啶酮光产物。
Nucleic Acids Res. 1992 Nov 11;20(21):5805-10. doi: 10.1093/nar/20.21.5805.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
8-(N-2-fluorenylacetamido)guanosine, an arylamidation reaction product of guanosine and the carcinogen N-acetoxy-N-2-fluorenylacetamide in neutral solution.8-（N-2-芴基乙酰胺基）鸟苷，鸟苷与致癌物N-乙酰氧基-N-2-芴基乙酰胺在中性溶液中的芳基酰胺化反应产物。
Biochemistry. 1967 Jan;6(1):177-82. doi: 10.1021/bi00853a029.
3
The interaction of caffeine with ultra-violet-light-irradiated DNA.咖啡因与紫外线照射的DNA之间的相互作用。
Int J Radiat Biol Relat Stud Phys Chem Med. 1970;17(4):395-9. doi: 10.1080/09553007014550481.
4
Proteins made in the mammalian cell cycle.哺乳动物细胞周期中产生的蛋白质。
J Biol Chem. 1971 Oct 25;246(20):6159-65.
5
Identity of corticosteroid binder I with the macromolecule binding 3-methylcholanthrene in liver cytosol in vivo.体内肝脏胞质溶胶中皮质类固醇结合蛋白I与结合3-甲基胆蒽的大分子的同一性。
Cancer Res. 1971 Oct;31(10):1364-8.
6
Lac repressor-operator interaction. I. Equilibrium studies.乳糖阻遏蛋白与操纵基因的相互作用。I. 平衡研究。
J Mol Biol. 1970 Feb 28;48(1):67-83. doi: 10.1016/0022-2836(70)90219-6.
7
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.聚乙二醇存在下噬菌体的快速沉降及其在大规模病毒纯化中的应用
Virology. 1970 Mar;40(3):734-44. doi: 10.1016/0042-6822(70)90218-7.
8
Carcinogenesis by aromatic amines.芳香胺致癌作用
Biochim Biophys Acta. 1974 Sep 9;355(2):177-203. doi: 10.1016/0304-419x(74)90003-1.
9
An endonuclease for depurinated DNA in rat liver.大鼠肝脏中一种作用于脱嘌呤DNA的核酸内切酶。
Can J Biochem. 1973 Jul;51(7):1003-9. doi: 10.1139/o73-130.
10
Properties of the endonuclease for depurinated DNA from Escherichia coli.来自大肠杆菌的脱嘌呤DNA的核酸内切酶特性。
Can J Biochem. 1972 Nov;50(11):1199-209. doi: 10.1139/o72-163.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验