Wilson D L, Hall M E, Stone G C
Gerontology. 1978;24(6):426-33. doi: 10.1159/000212282.
We have compared the proteins from superior cervical sympathetic ganglia (SCSG) in young and old male Fischer rats. We have searched for the kinds of changes in protein size or charge which are predicted by such cellular aging theories as the error catastrophe theory, the somatic mutation theory, the cross-linkage theory, translational aging, the deamidation theory, and the autoimmune theory. SCSG from young (2 months to 1 year) and old (21-24 months) rats were labeled in vitro for 1 h with 14C-leucine prior to protein extraction for analysis. Protein analysis was on high-resolution, two dimensional polyacrylamide gels using O'Farrell's technique, with some modifications. Proteins were separated according to isoelectric point in one dimension and molecular weight in the second dimension. The changes predicted by the above aging theories were detected neither in the staining pattern, showing protein amounts, nor in the labeling pattern on autoradiographs of dried gels, showing newly synthesized proteins. The results suggest the possibility that, at least for rat, some other mechanisms for aging are present.
我们比较了年轻和老年雄性费希尔大鼠颈上神经节（SCSG）中的蛋白质。我们探寻了蛋白质大小或电荷的变化类型，这些变化是由诸如误差灾难理论、体细胞突变理论、交联理论、翻译衰老、脱酰胺理论和自身免疫理论等细胞衰老理论所预测的。在提取蛋白质进行分析之前，将来自年轻（2个月至1年）和老年（21 - 24个月）大鼠的SCSG在体外用14C - 亮氨酸标记1小时。蛋白质分析采用O'Farrell技术在高分辨率二维聚丙烯酰胺凝胶上进行，并做了一些修改。蛋白质在一维中根据等电点分离，在二维中根据分子量分离。在显示蛋白质含量的染色模式中，以及在显示新合成蛋白质的干燥凝胶放射自显影片上的标记模式中，均未检测到上述衰老理论所预测的变化。结果表明，至少对于大鼠而言，存在其他一些衰老机制的可能性。
