Moore T L, Dorner R W
Arthritis Rheum. 1980 Nov;23(11):1262-7. doi: 10.1002/art.1780231106.
Fifty-four sera from children with juvenile rheumatoid arthritis (JRA) were separated by gel filtration at pH 4.05. The serum and IgM- and IgG-containing fractions were analyzed for heterophile antibodies by subjecting them to a hemolytic assay in tubes with the target cell a 1% washed sheep erythrocyte (SRBC) or beef erythrocyte (BRBC) in suspension. Thirty-three of 54 sera showed hemolytic antibody titers > 1:640 against SRBC alone and 4 of 54 had titers > 1:320 against SRBC and BRBC. All positive titers against SRBC and BRBC were associated with the IgM fraction. Differential absorption with SRBC, BRBC, and tissue sediment of homogenized guinea pig kidney revealed a Forssman specificity in 33 sera and Forssman and Hanganutziu-Deicher specificity in 4. The presence of heterophile antibodies in JRA correlated with the presence of hidden rheumatoid factor. There was also an increased incidence in patients with positive antinuclear antibodies and in teenaged females with polyarticular disease.
采用凝胶过滤法在pH 4.05条件下对54份青少年类风湿性关节炎（JRA）患儿的血清进行分离。通过在试管中用1%洗涤过的绵羊红细胞（SRBC）或悬浮的牛肉红细胞（BRBC）作为靶细胞进行溶血试验，对血清以及含IgM和IgG的组分进行嗜异性抗体分析。54份血清中有33份仅对SRBC的溶血抗体滴度>1:640，54份中有4份对SRBC和BRBC的滴度>1:320。所有针对SRBC和BRBC的阳性滴度均与IgM组分相关。用SRBC、BRBC和豚鼠肾匀浆组织沉淀物进行差异吸收试验显示，33份血清中有Forssman特异性，4份血清中有Forssman和Hanganutziu-Deicher特异性。JRA中嗜异性抗体的存在与隐匿性类风湿因子的存在相关。抗核抗体阳性的患者以及多关节疾病的青少年女性中发病率也有所增加。
