Tamura T, Kano K, Milgrom F
Diagn Immunol. 1984;2(2):101-4.
Mixed agglutination (MA) test with sediments of guinea pig kidney (GPK) homogenates and indicator red blood cells of bovine (BRBC) or sheep (SRBC) origin was established for detection of human heterophile antibodies. By means of MA test with BRBC indicator cells, heterophile antibodies of Hanganutziu-Deicher (H-D) specificity were demonstrated in sera of patients with syphilis (20%), lepromatous leprosy (57%), infectious mononucleosis (45%), Chediak-Higashi syndrome (73%), Kawasaki disease (58%), multiple sclerosis (58%), and leukemias (13%), as well as in sera of subjects who received injections of foreign species sera (20%). Some but not all BRBC-positive sera gave positive MA tests when SRBC were employed as indicator cells. None of 13 multiple myeloma sera tested gave positive results. The incidence of positive reactions in normal human sera was 3%. Neutralization of H-D antibodies in representative pathologic sera by purified heterophile antigens showed that the antibodies under investigation were mostly directed against antigen(s) of high molecular weight glycoprotein, but not N-glycolyl-neuraminic acid (NGNA) ganglioside fraction of BRBC.
建立了用豚鼠肾（GPK）匀浆沉淀物与牛（BRBC）或羊（SRBC）来源的指示红细胞进行混合凝集（MA）试验，以检测人嗜异性抗体。通过用BRBC指示细胞进行MA试验，在梅毒患者（20%）、瘤型麻风患者（57%）、传染性单核细胞增多症患者（45%）、切迪阿克-东综合征患者（73%）、川崎病患者（58%）、多发性硬化症患者（58%）和白血病患者（13%）的血清中，以及在接受异种血清注射的受试者（20%）的血清中，证实了具有汉-迪（H-D）特异性的嗜异性抗体。当使用SRBC作为指示细胞时，部分（但并非全部）BRBC阳性血清的MA试验呈阳性。所检测的13份多发性骨髓瘤血清均未得出阳性结果。正常人血清中的阳性反应发生率为3%。用纯化的嗜异性抗原中和代表性病理血清中的H-D抗体表明，所研究的抗体大多针对高分子量糖蛋白抗原，而非BRBC的N-羟乙酰神经氨酸（NGNA）神经节苷脂部分。
