Southwood T R, Roberts-Thomson P J, Ahern M J, Shepherd K, McEvoy R, Ziegler J B, Edmonds J
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, Adelaide, South Australia.
Ann Rheum Dis. 1990 Dec;49(12):968-72. doi: 10.1136/ard.49.12.968.
Antibodies to nuclear antigens were assessed in 23 children with juvenile chronic arthritis (JCA) and 66 of their first degree relatives. Serum samples from 16 patients with JCA (70%) and nine relatives (14%) had antinuclear antibodies by indirect immunofluorescence. Antibodies against nuclear antigens in rabbit thymus extract or an erythroblastoid cell line (K562) were detected by countercurrent immunoelectrophoresis and immunoblotting in 16 patients (70%) and 39 family relatives (59%). Immunoblotting did not show any banding patterns common to all patients with JCA, though bands in the 43-45 kD range were detected in 5/23 patients. Anticardiolipin antibodies were found in 7/23 patients. In total, 18/20 families (90%) had members other than the probands with detectable autoantibodies. In five families immunoblotting showed common banding patterns between the probands and other members. This suggests that there might be an inherited trend towards autoimmune responses in some families of patients with JCA.
对23名青少年慢性关节炎（JCA）患儿及其66名一级亲属进行了核抗原抗体评估。通过间接免疫荧光法，16例JCA患者（70%）和9名亲属（14%）的血清样本检测出抗核抗体。通过对流免疫电泳和免疫印迹法，在16例患者（70%）和39名家族亲属（59%）中检测到针对兔胸腺提取物或成红细胞样细胞系（K562）中核抗原的抗体。免疫印迹法未显示所有JCA患者共有的条带模式，不过在23例患者中的5例检测到43 - 45 kD范围内的条带。在23例患者中的7例发现了抗心磷脂抗体。总共，20个家族中有18个（90%）除先证者外还有其他成员可检测到自身抗体。在5个家族中，免疫印迹法显示先证者与其他成员之间有条带模式相同。这表明在一些JCA患者家族中可能存在自身免疫反应的遗传倾向。
