抗黄嘌呤氧化酶及牛乳脂肪球膜其他蛋白单克隆抗体的制备。
Preparation of monoclonal antibodies to xanthine oxidase and other proteins of bovine milk-fat-globule membrane.
作者信息
Mather I H, Nace C S, Johnson V G, Goldsby R A
出版信息
Biochem J. 1980 Jun 15;188(3):925-8. doi: 10.1042/bj1880925.
Abstract
Nine hybridomas secreting monoclonal antibody to proteins of bovine milk-fat-globule membrane were isolated. All nine cell lines continued to secrete monoclonal antibody after serial transfer in culture and after passage as solid tumours in Balb/cJ mice. Four of the cell lines secreted monoclonal antibody specific for xanthine oxidase, one of the major proteins of milk-fat-globule membrane.
摘要
分离出了9株分泌抗牛乳脂肪球膜蛋白单克隆抗体的杂交瘤。所有这9个细胞系在培养中连续传代以及作为实体瘤在Balb/cJ小鼠体内传代后仍继续分泌单克隆抗体。其中4个细胞系分泌对黄嘌呤氧化酶特异的单克隆抗体，黄嘌呤氧化酶是乳脂肪球膜的主要蛋白质之一。
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