Dilley W G, Birkhoff J D
Invest Urol. 1977 Jul;15(1):83-6.
Glandular benign prostatic hyperplasia specimens from 10 patients were culutured for 4 days in media containing either insulin alone, insulin plus estradiol, insulin plus testosterone, insulin plus human placental lactogen, insulin plus human placental lactogen plus testosterone, or insulin plus human growth hormone plus testosterone. Growth of the specimens was defined by pulsing 3H-thymidine on day 3, terminating on day 4, and determination of acid-insoluble radioactivity by scintillation counting. The response of each specimen was related to its base line response to insulin media. Six of nine specimens were stimulated 80 per cent or more by insulin plus placental lactogen plus testosterone, but only one of seven was stimulated by insulin plus testosterone. Four of 10 were stimulated by insulin plus estradiol. Histologic and autoradiographic results indicated that all growth occurred in the epithelium.
从10名患者的腺性良性前列腺增生标本在含有单独胰岛素、胰岛素加雌二醇、胰岛素加睾酮、胰岛素加人胎盘催乳素、胰岛素加人胎盘催乳素加睾酮或胰岛素加人生长激素加睾酮的培养基中培养4天。标本的生长通过在第3天脉冲3H-胸腺嘧啶核苷、在第4天终止培养并通过闪烁计数测定酸不溶性放射性来定义。每个标本的反应与其对胰岛素培养基的基线反应相关。9个标本中有6个受到胰岛素加胎盘催乳素加睾酮的刺激达80%或更高，但7个标本中只有1个受到胰岛素加睾酮的刺激。10个标本中有4个受到胰岛素加雌二醇的刺激。组织学和放射自显影结果表明，所有生长均发生在上皮细胞中。
