Fogelmark B, Rylander R, Sjöstrand M, Reininghaus W
Exp Lung Res. 1980 Jun;1(2):131-8. doi: 10.3109/01902148009069643.
We report on a technique for studying phagocytosis in free lung cells with the use of fungal spores. Free lung cells were obtained from a bronchial lavage. They were incubated with fungal spores and the engulfment of these spores was studied at various time intervals and under different conditions. The phagocytosis process was found to occur from relatively stationary macrophages within the first hours after incubation. The number of engulfed spores was proportional to their number in the solution. Addition of serum or surfactant to the medium increased the phagocytosis rate. In hamsters and rats exposed to tobacco smoke under in vivo conditions, a dose-related increase in phagocytosis rate could be demonstrated.
我们报告了一种利用真菌孢子研究游离肺细胞吞噬作用的技术。游离肺细胞取自支气管灌洗。将它们与真菌孢子一起孵育，并在不同时间间隔和不同条件下研究这些孢子的吞噬情况。发现吞噬过程在孵育后的最初几个小时内由相对静止的巨噬细胞发生。吞噬的孢子数量与溶液中它们的数量成正比。向培养基中添加血清或表面活性剂可提高吞噬率。在体内条件下暴露于烟草烟雾的仓鼠和大鼠中，可证明吞噬率呈剂量相关增加。
