Rylander R, Fogelmark B, Sjöstrand M
Agents Actions. 1985 Jul;16(5):353-8. doi: 10.1007/BF01982872.
Free lung cells were studied in guinea-pigs different times after an inhalation exposure to bacterial endotoxin yielding a dose of about 1 microgram/animal. After exposure the number of alveolar macrophages (AM) and neutrophils was increased. The phagocytosis capacity of the lung was increased, mainly due to the influx of neutrophils. The activity of N-acetyl-beta-D-glucosaminidase from exposed animals was decreased in AM cell cultures and increased in lavage fluid 2 hours and later after exposure. Cathepsin D activity was also decreased in AM cell cultures but did not change in lavage fluid. Lactate dehydrogenase was increased in lung lavage fluid. The results show that LPS inhalation activates AM and that increased amounts of lysosomal enzymes are present in the airways.
在豚鼠吸入剂量约为1微克/只的细菌内毒素后的不同时间，对其游离肺细胞进行了研究。暴露后，肺泡巨噬细胞（AM）和中性粒细胞数量增加。肺的吞噬能力增强，主要是由于中性粒细胞的流入。暴露动物的N-乙酰-β-D-氨基葡萄糖苷酶活性在AM细胞培养物中降低，而在暴露后2小时及之后的灌洗液中升高。组织蛋白酶D活性在AM细胞培养物中也降低，但在灌洗液中没有变化。肺灌洗液中的乳酸脱氢酶升高。结果表明，吸入脂多糖可激活AM，并且气道中存在数量增加的溶酶体酶。
