烟草花叶病毒蛋白可诱导脂质体膜融合。

Tobacco mosaic virus protein induces fusion of liposome membranes.

作者信息

Banerjee S, Vandenbranden M, Ruysschaert J M

出版信息

Biochim Biophys Acta. 1981 Aug 20;646(2):360-4. doi: 10.1016/0005-2736(81)90345-x.

Abstract

The fusogenic properties of tobacco mosaic virus (TMV) coat protein were investigated. Tobacco mosaic virus protein induces membrane fusion of a population of L-alpha-dimyristoylphosphatidylcholine (DMPC) and DL-alpha-dipalmitoylphosphatidylcholine (DPPC) vesicles giving rise to larger particles as seen by a drastic absorbance increase of the liposomal solution. Differential scanning calorimetry spectra demonstrate complete mixing of the acyl chains of the lipids during fusion. Electron micrographs indicate that the fused entities are multilamellar.

摘要

对烟草花叶病毒（TMV）外壳蛋白的融合特性进行了研究。烟草花叶病毒蛋白可诱导一群L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）和DL-α-二棕榈酰磷脂酰胆碱（DPPC）囊泡发生膜融合，形成更大的颗粒，这可通过脂质体溶液吸光度的急剧增加得以观察到。差示扫描量热法光谱表明，融合过程中脂质的酰基链完全混合。电子显微镜照片显示，融合后的实体是多层的。