Mason W T, Lane N J, Miller N G, Bangham A D
J Membr Biol. 1980 Jun 30;55(1):69-79. doi: 10.1007/BF01926370.
It has been found that the n-alkyl bromides are capable of inducing the fusion of unilamellar liposomes. These compounds can bring about fusion of liposomes composed of either pure phosphatidylcholine or phosphatidycholine+phosphatidic acid. Fusion of unilamellar liposomes gives rise to multilamellar structures, the morphology of which has been examined by negative staining and freeze-fracture techniques. It has been shown by microelectrophoresis that the n-alkyl bromides have no effect on the surface charge of liposomes, and fusion has been further characterized by use of light scattering and differential scanning calorimetry, the latter indicating that true mixing of the fatty acyl chains occurs upon fusion. Finally, fusion occurs at n-alkyl bromide levels below that required to saturate the aqueous phase of the system.
已发现正烷基溴化物能够诱导单层脂质体融合。这些化合物可促使由纯磷脂酰胆碱或磷脂酰胆碱 + 磷脂酸组成的脂质体发生融合。单层脂质体的融合会产生多层结构，其形态已通过负染色和冷冻断裂技术进行了检测。微电泳表明正烷基溴化物对脂质体的表面电荷没有影响，并且已通过光散射和差示扫描量热法对融合进行了进一步表征，后者表明融合时脂肪酰链发生了真正的混合。最后，在低于使系统水相饱和所需的正烷基溴化物水平时就会发生融合。
