用矩量法分析非指数荧光衰减数据。
Analysis of nonexponential fluorescence decay data by a method of moments.
作者信息
Solie T N, Small E W, Isenberg I
出版信息
Biophys J. 1980 Mar;29(3):367-78. doi: 10.1016/S0006-3495(80)85140-X.
Abstract
A method of moments is presented for the analysis of convoluted fluorescence decay data when the impulse response function is given by f(t) = alpha exp (-At - Bt1/2). Examples of this method are given using both simulated and measured fluorescence decays. It is also shown that this method, used with moment index displacements, will correct for light-scatter leakage, zero-point time shifts, and slow lamp drift.
摘要
当脉冲响应函数由f(t)=αexp(-At - Bt1/2)给出时，提出了一种矩量法用于分析卷积荧光衰减数据。使用模拟和测量的荧光衰减给出了该方法的示例。还表明，该方法与矩指数位移一起使用时，将校正光散射泄漏、零点时间偏移和慢灯漂移。
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3
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