通过拉普拉斯变换分析荧光衰减曲线。
Analysis of fluorescence decay curves by means of the Laplace transformation.
作者信息
Gafni A, Modlin R L, Brand L
出版信息
Biophys J. 1975 Mar;15(3):263-80. doi: 10.1016/S0006-3495(75)85817-6.
Abstract
A computational procedure is described for the analysis of fluorescence decay data convolved with a lamp flash of finite width. The computer program calculates the ratio of the Laplace transforms of the decay and the lamp flash for different values of s to give the transforms of the impulse response for each value of s. These are set equal to the analytical Laplace transforms of the decay law involved. Solution of the nonlinear simultaneous equations yields the desired decay parameters. The method can be modified to analyze data that contains a component due to scattered light and can also provide essential information regarding transit time changes of the photomultiplier with changes in emission wavelength. The method was tested by the analysis of real and simulated data. The accuracy of the analysis depends on the degree of correlation among the parameters.
摘要
描述了一种用于分析与有限宽度灯闪光卷积的荧光衰减数据的计算程序。该计算机程序针对不同的s值计算衰减和灯闪光的拉普拉斯变换的比值，以给出每个s值的脉冲响应变换。将这些变换设置为等于所涉及衰减定律的解析拉普拉斯变换。求解非线性联立方程可得到所需的衰减参数。该方法可以修改以分析包含散射光成分的数据，并且还可以提供关于光电倍增管的渡越时间随发射波长变化的基本信息。通过对真实数据和模拟数据的分析对该方法进行了测试。分析的准确性取决于参数之间的相关程度。
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