紫外线照射的噬菌体T4中的切除修复与补丁大小
Excision repair and patch size in UV-irradiated bacteriophage T4.
作者信息
Yarosh D B, Rosenstein B S, Setlow R B
出版信息
J Virol. 1981 Nov;40(2):465-71. doi: 10.1128/JVI.40.2.465-471.1981.
Abstract
We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control experiments with the denV1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA and prevented nicking by the UV-specific endonucleases of Micrococcus luteus and bacteriophage T4.
摘要
我们通过测量修复过程中掺入的溴脱氧尿苷的光解作用，确定了噬菌体T4中紫外线损伤修复时切除修复片段的平均大小。平均片段很小，大约四个核苷酸长。在用denV1切除缺陷型突变体进行的对照实验中，我们遇到了一个假象，一种蛋白质仍然与苯酚提取的DNA结合，并阻止了藤黄微球菌和噬菌体T4的紫外线特异性内切核酸酶进行切口。
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