噬菌体T4对紫外线照射的脱氧核糖核酸的暗修复：一种二聚体特异性噬菌体诱导的内切核酸酶的纯化与特性分析
Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.
作者信息
Friedberg E C, King J J
出版信息
J Bacteriol. 1971 May;106(2):500-7. doi: 10.1128/jb.106.2.500-507.1971.
Abstract
The purification and properties of an ultraviolet (UV) repair endonuclease are described. The enzyme is induced by infection of cells of Escherichia coli with phage T4 and is missing from extracts of cells infected with the UV-sensitive and excision-defective mutant T4V(1). The enzyme attacks UV-irradiated deoxyribonucleic acid (DNA) containing either hydroxymethylcytosine or cytosine, but does not affect native DNA. The specific substrate in UV-irradiated DNA appears to be pyrimidine dimer sites. The purified enzyme alone does not excise pyrimidine dimers from UV-irradiated DNA. However, dimer excision does occur in the presence of the purified endonuclease plus crude extract of cells infected with the mutant T4V(1).
摘要
本文描述了一种紫外线（UV）修复内切核酸酶的纯化及特性。该酶由噬菌体T4感染大肠杆菌细胞诱导产生，而感染对紫外线敏感且切除缺陷型突变体T4V(1)的细胞提取物中则没有这种酶。该酶可作用于含有羟甲基胞嘧啶或胞嘧啶的紫外线照射过的脱氧核糖核酸（DNA），但不影响天然DNA。紫外线照射过的DNA中的特异性底物似乎是嘧啶二聚体位点。单独的纯化酶并不能从紫外线照射过的DNA中切除嘧啶二聚体。然而，在纯化的内切核酸酶加上感染突变体T4V(1)的细胞粗提取物存在的情况下，确实会发生二聚体切除。
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