Notter R H, Shapiro D L, Taubold R, Chen J
Pediatr Res. 1982 Feb;16(2):130-6. doi: 10.1203/00006450-198202000-00011.
This work examines the dynamic surface pressure-area behavior of films of unconjugated bilirubin spread from chloroform solution at 22 degrees C on 0.15 M NaCl and buffered phosphate subphases. Film behavior is examined at pH 5.5, 7.4 and 8.0. The interactions of bilirubin in mixed films with dipalmitoyl phosphatidylcholine and with 9:1 dipalmitoyl phosphatidylcholine:dioleolyl phosphatidylcholine are examined at similar temperature and pH values. It is found that unconjugated bilirubin modifies the dynamic surface pressure-area behavior of phospholipid films in both the high and low surface pressure regimes, with bilirubin exerting its greatest effect at low pH where its subphase solubility is low. Because many premature infants suffering from the Respiratory Distress Syndrome (RDS) have accompanying hyperbilirubinemia, with possible bilirubin transport to the alveolar space, the interactions of bilirubin with phospholipid films are discussed in terms of potential effects on pulmonary surfactant in vivo.
本研究考察了未结合胆红素从氯仿溶液在22℃下铺展于0.15M氯化钠和磷酸盐缓冲亚相上形成的薄膜的动态表面压力-面积行为。在pH值为5.5、7.4和8.0条件下研究薄膜行为。在相似温度和pH值下，考察了未结合胆红素在混合薄膜中与二棕榈酰磷脂酰胆碱以及9:1的二棕榈酰磷脂酰胆碱:二油酰磷脂酰胆碱的相互作用。结果发现，未结合胆红素在高表面压力和低表面压力区域均改变了磷脂薄膜的动态表面压力-面积行为，胆红素在低pH值（此时其在亚相中的溶解度低）时发挥最大作用。由于许多患有呼吸窘迫综合征（RDS）的早产儿伴有高胆红素血症，胆红素可能转运至肺泡腔，因此从胆红素与磷脂薄膜的相互作用对体内肺表面活性剂的潜在影响方面进行了讨论。
