从亚急性硬化性全脑炎脑部分离的核衣壳组分中麻疹病毒样RNA的富集。

Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains.

作者信息

Bedows E, Kohne D E, Tourtellotte W W, Payne F E

出版信息

J Virol Methods. 1982 Feb;4(1):37-43. doi: 10.1016/0166-0934(82)90052-0.

Abstract

A procedure has been developed which facilitates the detection of measles virus RNA sequences in human brains. The procedure involves isolating subviral components (nucleocapsids) from brain tissues prior to RNA purification, followed by hybridization of these RNAs to cDNA synthesized from measles virus 50 S RNA template. Using these techniques we were able to obtain an RNA fraction which was manyfold enriched in measles virus-specific RNA, relative to unfractionated subacute sclerosing panencephalitis (SSPE) brain RNAs 70-100% of the measles virus-specific RNA present these SSPE brain samples were recovered in this enriched fraction.

摘要

已开发出一种有助于检测人脑中麻疹病毒RNA序列的方法。该方法包括在RNA纯化之前从脑组织中分离亚病毒成分（核衣壳），然后将这些RNA与由麻疹病毒50S RNA模板合成的cDNA进行杂交。使用这些技术，我们能够获得一个RNA组分，相对于未分级的亚急性硬化性全脑炎（SSPE）脑RNA，该组分中麻疹病毒特异性RNA富集了许多倍。在这些SSPE脑样本中存在的70 - 100%的麻疹病毒特异性RNA在这个富集组分中被回收。