Dowling P C, Blumberg B M, Kolakofsky D, Cook P, Jotkowitz A, Prineas J W, Cook S D
Virus Res. 1986 Jul;5(1):97-107. doi: 10.1016/0168-1702(86)90068-7.
We constructed a measles virus genomic recombinant DNA library, and used clones coding for portions of the viral P, M and H proteins to probe for measles virus nucleic acid sequences in post-mortem multiple sclerosis, SSPE and control brains. By dot blot hybridization, the probes detected measles virus nucleic acid sequences in as little as 3 nanograms of total RNA extracted from measles virus-infected cells and also in highly diluted RNA extracted from SSPE brain, but did not detect measles virus sequences in RNA extracted from 11 multiple sclerosis or 8 control brains, even at a 1 000-fold higher concentration of RNA. By in situ hybridization, these probes detected measles virus nucleic acid sequences in virtually every cell and the surrounding neuropile of SSPE brain, but again did not detect such sequences in multiple sclerosis or control brains. Our findings using these highly specific probes confirm that measles virus is found in SSPE brains and indicate that measles virus genome is unlikely to be present in multiple sclerosis or normal brains.
我们构建了一个麻疹病毒基因组重组DNA文库，并使用编码病毒P、M和H蛋白部分片段的克隆来探测尸检的多发性硬化症、亚急性硬化性全脑炎（SSPE）及对照大脑中的麻疹病毒核酸序列。通过斑点印迹杂交，这些探针在从感染麻疹病毒的细胞中提取的低至3纳克总RNA中检测到了麻疹病毒核酸序列，在从SSPE大脑中提取的高度稀释的RNA中也检测到了，但即使在RNA浓度高出1000倍的情况下，从11例多发性硬化症患者或8例对照大脑中提取的RNA中也未检测到麻疹病毒序列。通过原位杂交，这些探针在几乎SSPE大脑的每个细胞及周围神经纤维网中都检测到了麻疹病毒核酸序列，但同样在多发性硬化症或对照大脑中未检测到此类序列。我们使用这些高度特异性探针的研究结果证实，在SSPE大脑中发现了麻疹病毒，并表明麻疹病毒基因组不太可能存在于多发性硬化症或正常大脑中。
