Berger R E, Karp L E, Williamson R A, Koehler J, Moore D E, Holmes K K
Fertil Steril. 1982 Apr;37(4):557-64. doi: 10.1016/s0015-0282(16)46166-2.
The relationship of the parameters of seminal fluid analysis (SFA) and seminal fluid leukocyte concentration to the in vitro sperm penetration assay (SPA) was studied in 217 men from infertile couples. Significant positive correlations were found between the SPA and sperm count, sperm motility, and morphologically normal sperm. The total number of leukocytes per milliliter, the number of polymorphonuclear leukocytes per milliliter (PMN), and the number of lymphocytes per milliliter were negatively correlated with the results of the SPA. The strongest correlation with an abnormal SPA (less than 11% of eggs penetrated) was the presence of greater than or equal to 1 PMN/100 sperm (8.17 increased relative risk of an abnormal SPA). The most important predictor variables of the percent penetration of the sperm penetration assay were total numbers of leukocytes per milliliter, sperm count, and sperm motility. Microbiologic studies of semen were not correlated with SPA results or number of leukocytes in the semen.
对217对不育夫妇中的男性进行研究，以探讨精液分析（SFA）参数及精液白细胞浓度与体外精子穿透试验（SPA）之间的关系。结果发现，SPA与精子计数、精子活力及形态正常精子之间存在显著正相关。每毫升白细胞总数、每毫升多形核白细胞（PMN）数量及每毫升淋巴细胞数量与SPA结果呈负相关。与异常SPA（卵子穿透率低于11%）相关性最强的是每100个精子中PMN≥1个（SPA异常的相对风险增加8.17倍）。精子穿透试验穿透率的最重要预测变量是每毫升白细胞总数、精子计数及精子活力。精液的微生物学研究与SPA结果或精液中的白细胞数量无关。
