Tereshchenko M I, Grishchenko A M, Mashkovskiĭ N N, Kaliushko L I
Ukr Biokhim Zh (1978). 1982 May-Jun;54(3):311-5.
The DNA-binding activity of blood serum proteins was determined in the process of the immune response accompanied and not accompanied by the appearance of antibodies to DNA in blood immunized animals. The immunological rearrangements in the organism following DNA administration without the appearance of antibodies against a unihelical DNA in the blood serum cause an increase in the DNA-binding ability of blood serum proteins, which decreases the specificity of the radioimmunological method in determination of antibodies to DNA. Denaturation of DNA in the presence of formalin also increases the nonspecific binding with DNA of blood serum proteins containing and noncontaining antibodies to DNA.
在免疫反应过程中，测定了免疫动物血清蛋白的DNA结合活性，该免疫反应伴有或不伴有血液中抗DNA抗体的出现。在给予DNA后机体发生免疫重排，但血清中未出现针对单螺旋DNA的抗体，这会导致血清蛋白的DNA结合能力增加，从而降低放射免疫法检测抗DNA抗体的特异性。在福尔马林存在的情况下，DNA变性也会增加血清中含或不含抗DNA抗体的蛋白质与DNA的非特异性结合。
