Lechan R M, Nestler J L, Jacobson S
Brain Res. 1982 Aug 5;245(1):1-15. doi: 10.1016/0006-8993(82)90334-1.
The origin of neuronal perikarya which project to the external zone of the median eminence (the tuberoinfundibular neuronal system) was determined in the rat after injection or diffusion of wheat germ agglutinin (WGA) into the median eminence. The retrogradely transported lectin was detected in neurons using an immunohistochemical method based on the peroxidase-antiperoxidase technique. Immunoreactive cell bodies were found both in hypothalamic and extrahypothalamic regions. Within the hypothalamus, the majority of peroxidase-positive cells were present in the dorsomedial and basolateral portions of the arcuate nucleus, regions of the periventricular nucleus, and the preoptic region, particularly at the level of the organum vasculosum of the lamina terminalis (OVLT). Within the extrahypothalamic regions, WGA-positive perikarya were found in the diagonal band of Broca, the region of the medical septum and the brainstem. Only rare cells were labeled in the ventromedial nucleus of the hypothalamus and no cells were labeled in any region of the amygdala. These data demonstrate that neurons with afferent projections to the median eminence are more widely distributed in the rat brain than previously recognized and therefore, that the concept of the tuberoinfundibular neuronal system must be expanded.
将小麦胚芽凝集素（WGA）注射到大鼠正中隆起或使其在正中隆起扩散后，确定了投射至正中隆起外侧区（结节漏斗神经元系统）的神经元胞体的起源。使用基于过氧化物酶-抗过氧化物酶技术的免疫组织化学方法，在神经元中检测到逆行运输的凝集素。在下丘脑和下丘脑外区域均发现了免疫反应性细胞体。在下丘脑内，大多数过氧化物酶阳性细胞存在于弓状核的背内侧和基底外侧部分、室周核区域以及视前区，特别是终板血管器（OVLT）水平。在下丘脑外区域，在布罗卡斜带、内侧隔区和脑干中发现了WGA阳性胞体。在下丘脑腹内侧核中仅发现极少数细胞被标记，杏仁核的任何区域均未发现细胞被标记。这些数据表明，向正中隆起有传入投射的神经元在大鼠脑中的分布比以前认为的更广泛，因此，结节漏斗神经元系统的概念必须扩大。
